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Series GSE233924 Query DataSets for GSE233924
Status Public on Jun 06, 2023
Title Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells [Toxicants]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Animal studies for embryotoxicity evaluation of potential therapeutics and environmental factors are complex, costly, and time-consuming. Often, studies are not of human relevance because of species differences. In the present study, we recapitulated the process of cardiomyogenesis in human induced pluripotent stem cells (hiPSCs) by modulation of the Wnt signaling pathway to identify a key cardiomyogenesis gene signature that can be applied to identify compounds and/or stress factors compromising the cardiomyogenesis process.
Among the 23 tested teratogens and 16 non-teratogens, we identified three retinoids including 13-cis-retinoic acid that completely block the process of cardiomyogenesis in hiPSCs. Moreover, we have identified an early gene signature consisting of 31 genes and associated biological processes that are severely affected by the retinoids. To predict the inhibitory potential of teratogens and non-teratogens in the process of cardiomyogenesis we established the “Developmental Cardiotoxicity Index” (DCI_31g) that accurately differentiates teratogens and non-teratogens to do or do not affect the differentiation of hiPSCs to functional cardiomyocytes.
Overall design Human iPSCs wered differentiated for 14 days according to a cardiomyogenic protocol and incubated for 24 h at day 1 with compounds at the same time. RNA was isolated from these cells, at spesific timepoints and a whole transcritpome analysis was performed. Compound-induced gene expression changes were determined with statistical and biological evalutation.
Contributor(s) Cherianidou A, Kappenberg F, Seidel F, Papazoglou P, Srinivasan SP, Leist M, Hengstler JG, Rahnenführer J, Sachinidis A
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Submission date Jun 01, 2023
Last update date Sep 05, 2023
Contact name Agapios Sachinidis
Phone +492214787373
Organization name University of Cologne
Department Institute of Neurophysiology
Street address Robert-Kochstr. 39
City Cologne
State/province NRW
ZIP/Postal code 50931
Country Germany
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (57)
GSM7439138 UKK2_hiPSC_untreated_Day 0_R1
GSM7439139 UKK2_hiPSC_untreated_Day 0_R2
GSM7439140 UKK2_hiPSC_untreated_Day 0_R3
This SubSeries is part of SuperSeries:
GSE233926 Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells
BioProject PRJNA978589

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE233924_RAW.tar 252.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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