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| Status |
Public on Jun 07, 2023 |
| Title |
Transcriptional chracterization of ABCC1 and ABCB1 KO and MZ1 treated WT HAP1 cell lines. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used CRISPR/Cas9 to intoroduce frame-shifting mutations into the ABCC1 and ABCB1 genes and treated HAP1 WT cells with varying concentrations of the MZ1 PROTAC. To measure the transcriptional response to both genetic and chemical perturbances, we performed RNA-seq and quantified gene expression levels.
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| Overall design |
6 cell lines, untreated or MZ1 treated in 1-3 technical replicates
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| Contributor(s) |
Wolf G, Onstein S, Christodoulaki E, Superti-Furga G |
| Citation(s) |
37516113 |
| |
| Submission date |
Jun 02, 2023 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Giulio Superti-Furga |
| Organization name |
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
|
| Street address |
Lazarettgasse 14
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA979232 |
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