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| Status |
Public on Jun 09, 2023 |
| Title |
CTLA-4 tail fusion enhances CAR-T anti-tumor immunity [single-cell RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To systematically investigate the effect of CCT engineering on CAR-T cell phenotypes in vivo, we used single-cell RNA sequencing (scRNA-seq) to characterize the full spectrum of engineered CAR-T cells and their transcriptomic profiles
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| Overall design |
NSG mice were inoculated with 0.5 million NALM6GL cells intravenously (i.v.) on day 0 and treated with 1 million CAR-T cells (i.v.) on day 4. Relapse was modeled by re-challenging all mice with 0.5 million NALM6GL cells(i.v.) on day 12. To better model the clinical response to CAR-T cells in NSG mice45, 2.5µg human recombinant IL2 (Peprotech) was administered subcutaneously (s.c.) everyday starting on from day 4 to day 14. All mice were sacrificed on day 15. Spleen and bone marrow tissues were collected and processed into single cell suspension. CAR-T cells were sorted using Aria II cell sorter (BD) based on mScarlet expression. For all four groups (CAR, CAR-1CCT, CAR-2CCT and CAR-3CCT), samples from 3 individual mice within the same group were pooled together to minimize sampling bias.
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| Contributor(s) |
Zhou X, Cao H, Renauer P, Chen S |
| Citation(s) |
37500885 |
| |
| Submission date |
Jun 07, 2023 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Sidi Chen |
| E-mail(s) |
sidi.chen@yale.edu
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| Organization name |
Yale University
|
| Street address |
850 West Campus Drive
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| City |
West Haven |
| State/province |
CT |
| ZIP/Postal code |
06516 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE234307 |
CTLA-4 tail fusion enhances CAR-T anti-tumor immunity |
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| Relations |
| BioProject |
PRJNA981104 |
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