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Series GSE234347 Query DataSets for GSE234347
Status Public on Jun 09, 2023
Title CTLA-4 tail fusion enhances CAR-T anti-tumor immunity [single-cell RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To systematically investigate the effect of CCT engineering on CAR-T cell phenotypes in vivo, we used single-cell RNA sequencing (scRNA-seq) to characterize the full spectrum of engineered CAR-T cells and their transcriptomic profiles
 
Overall design NSG mice were inoculated with 0.5 million NALM6GL cells intravenously (i.v.) on day 0 and treated with 1 million CAR-T cells (i.v.) on day 4. Relapse was modeled by re-challenging all mice with 0.5 million NALM6GL cells(i.v.) on day 12. To better model the clinical response to CAR-T cells in NSG mice45, 2.5µg human recombinant IL2 (Peprotech) was administered subcutaneously (s.c.) everyday starting on from day 4 to day 14. All mice were sacrificed on day 15. Spleen and bone marrow tissues were collected and processed into single cell suspension. CAR-T cells were sorted using Aria II cell sorter (BD) based on mScarlet expression. For all four groups (CAR, CAR-1CCT, CAR-2CCT and CAR-3CCT), samples from 3 individual mice within the same group were pooled together to minimize sampling bias.
 
Contributor(s) Zhou X, Cao H, Renauer P, Chen S
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Submission date Jun 07, 2023
Last update date Jun 11, 2023
Contact name Sidi Chen
E-mail(s) sidi.chen@yale.edu
Organization name Yale University
Street address 850 West Campus Drive
City West Haven
State/province CT
ZIP/Postal code 06516
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (8)
GSM7465439 SP-39a
GSM7465440 SP-39b
GSM7465441 SP-39c
This SubSeries is part of SuperSeries:
GSE234307 CTLA-4 tail fusion enhances CAR-T anti-tumor immunity
Relations
BioProject PRJNA981104

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE234347_RAW.tar 356.4 Mb (http)(custom) TAR (of H5)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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