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Series GSE234940 Query DataSets for GSE234940
Status Public on Jun 14, 2024
Title Blood miRNA as biomarkers of epilepsy (EARLY vs. LATE)
Platform organism synthetic construct
Sample organism Rattus norvegicus
Experiment type Non-coding RNA profiling by array
Summary Epilepsy frequently develops as a result of brain insult, for example brain injury or stroke. Currently there are no tools allowing us to predict which trauma patients will eventually develop epilepsy. There is evidence that microRNAs levels are altered in the blood, making them attractive candidates for peripheral biomarkers of epilepsy.
We analyzed miRNA levels in blood samples using Affymetrix microarrays 4.1 and performed comparative analysis of samples. We performed 3 comparisons: i) control animals vs. Status epilepticus animals, ii) animals which developed first spontaneous seizure around 7 days post stimulation (EARLY), or later after 21days post stimulation (LATE), iii) animals that had high or low number of seizures as determined by seizures number (EARLY vs. LATE).
 
Overall design We draw the blood from rats after electrical stimulation of amygdala, which induces status epilepticus, at 3 timepoints: 2 days, 9 days and 8 months. Laterr RNA was isolated from all the samples at the same time and microarray 4.1 were used in agreement with the producer's protocol, to test levels of each miRNA.
Web link https://www.biorxiv.org/content/10.1101/2024.02.29.582734v1
 
Contributor(s) Szydlowska K, Lukasiuk K
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 14, 2023
Last update date Jun 18, 2024
Contact name Kinga Szydlowska
E-mail(s) kinga.szydlowska@gmail.com
Phone +48225892152
Organization name Nencki Institute of Experimental Biolo
Lab Laboratory of Epileptogenesis
Street address Pasteura 3
City Warsaw
State/province Select State
ZIP/Postal code 02-093
Country Poland
 
Platforms (1)
GPL21572 [miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version]
Samples (45)
GSM7483329 blood sample from control rat (EARLY vs. LATE) at 2 days, biological replicate 1
GSM7483330 blood sample from control rat (EARLY vs. LATE) at 2 days, biological replicate 2
GSM7483332 blood sample from control rat (EARLY vs. LATE) at 2 days, biological replicate 3
This SubSeries is part of SuperSeries:
GSE234942 Blood miRNA as biomarkers of epilepsy
Relations
BioProject PRJNA983780

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE234940_RAW.tar 33.2 Mb (http)(custom) TAR (of CEL)
GSE234940_RMA.txt.gz 290.7 Kb (ftp)(http) TXT
Processed data are available on Series record

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