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| Status |
Public on Dec 13, 2023 |
| Title |
Single nucleus multiome (RNAseq and ATACseq) of male mouse pituitary |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the single nucleus multiome (RNAseq+ATACseq) of a male mouse pituitary sample. This dataset was generated for supporting the development of a novel computational framework for analyzing gene regulatorty circuitry from single cell multiome datasets. This computational framework extract regulatory circuits consisting of TFs, cis-regulatory sites and target genes by modelling the co-incidence of the RNA levels of the TFs, the chomatin accessibility of the TFs' binding sites and the RNA levels of target genes across single cell. Specifically we identified gonadotrope-specific regulatory circuits under the transcription factor Gata2. These regulatory circuits were validated by male mouse pituitary samples with gonadotrope-conditional knockout of Gata2 previously reported in GSE190066.
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| Overall design |
Single nucleus true multiome (RNAseq and ATACseq) of the pituitary tissue of a male mouse.
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| Contributor(s) |
Zhang Z, Ruf-Zamojski F, Zamojski M, Natalia M, Bernard D, Sealfon S |
| Citation(s) |
38084892 |
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| Submission date |
Jun 14, 2023 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Michel A Zamojski |
| E-mail(s) |
michel.zamojski@cshs.org
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| Organization name |
Cedars-Sinai Medical Center
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| Department |
Regenerative Medicine Institute
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| Lab |
Svendsen
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| Street address |
8700 Beverly Boulevard
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| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90048 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA983783 |
