|
| Status |
Public on Jun 22, 2023 |
| Title |
Human HFF cells large rearranements detection after CRISPR-Cas9 nuclease (Fig.2c) |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by genome tiling array
Genome variation profiling by SNP array
|
| Summary |
ABRAXAS2 gene editing by CRISPR-Cas9 nuclease in heterozygous HFF for UROS inactivation. Fluorescent positive cell sorting for UROS and large rearrangements confirmation in chromosome 10q.
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| |
|
| Overall design |
Three-condition experiment,edited HFF vs Agilent control DNA or control HFF. Fluorescent positive cell bulk and two fluorescent-positive clones, #1 and #2 after ABRAXAS2 gene editing.
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| |
|
| Contributor(s) |
Boutin J, Pennamen P |
| Citation(s) |
37429857 |
| |
| Submission date |
Jun 15, 2023 |
| Last update date |
Sep 12, 2023 |
| Contact name |
Julian Boutin |
| E-mail(s) |
julian.boutin@u-bordeaux.fr
|
| Organization name |
INSERM U1312 BRIC
|
| Lab |
BioGO team 8
|
| Street address |
146 rue Leo Saignat
|
| City |
Bordeaux |
| State/province |
Nouvelle Aquitaine |
| ZIP/Postal code |
33000 |
| Country |
France |
| |
|
| Platforms (2) |
| GPL17052 |
Agilent-021924 SurePrint G3 Human CGH Microarray 8x60K (Probe Name version) |
| GPL33494 |
Agilent-085591 Human 180K CGH+SNP |
|
| Samples (3) |
| GSM7493282 |
HFF ABRAXAS2 transfected CGH bulk |
| GSM7493283 |
HFF ABRAXAS2 transfected CGH clone 1 (del terminale) |
| GSM7493284 |
HFF ABRAXAS2 transfected CGH clone 3 (CN + dup) |
|
| Relations |
| BioProject |
PRJNA984190 |
