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Series GSE23601 Query DataSets for GSE23601
Status Public on Nov 21, 2012
Title gene expression profiling of brain metastatic melanoma cell lines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cerebral metastases occur in a majority of metastatic melanoma patients and are a major cause of mortality. Despite this, there is a poor understanding of the molecules/pathways that lead to the brain-metastatic phenotype. Studies designed to address this deficiency and test novel therapeutic approaches have until recently been slowed by an absence of preclinical models of spontaneous CNS metastatic melanoma disease. To address this, we isolated two variants of the human melanoma cell line WM239 (named 131/4-5B1 and 131/4-5B2) which can metastasize spontaneously to brain parenchyma from an orthotopic primary transplant. We have performed gene expression profiling on both brain metastatic cell lines (131/4-5B1 and 131/4-5B2) and compared to the poorly metastatic parental cell line WM239A and a derived highly metastatic variant 113/6-4L in order to examine the mechanisms that influence the progression of malignant melanoma to a brain-metastatic phenotype.
 
Overall design Two-condition experiment, brain metastatic cell lines (131/4-5B1 and 131/4-5B2) and compared to the poorly metastatic parental cell line WM239A and a derived highly metastatic variant 113/6-4L. Biological replicates: 4 independently grown and harvested cell line passages. Two technical replicate per condition (including dye swap).
 
Contributor(s) Jaramillo ML, Nantel A, Cruz-Muñoz W
Citation(s) 22865454
Submission date Aug 12, 2010
Last update date Nov 22, 2012
Contact name Andre Nantel
E-mail(s) andre.nantel@nrc-cnrc.gc.ca
Phone 514-496-6370
Fax 514-496-9127
Organization name National Research Council of Canada
Department Biotechnology Research Institute
Street address 6100 Royalmount
City Montreal
State/province QC
ZIP/Postal code H4P 2R2
Country Canada
 
Platforms (1)
GPL10796 NRC-BRI Human HEEBO 39K array
Samples (20)
GSM578854 metastatic 113/6-4L vs poorly metastatic WM239 replicate #1
GSM578855 metastatic 113/6-4L vs poorly metastatic WM239 replicate #2
GSM578856 metastatic 113/6-4L vs poorly metastatic WM239 replicate #3
Relations
BioProject PRJNA129959

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23601_RAW.tar 80.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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