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Series GSE236262 Query DataSets for GSE236262
Status Public on Jun 30, 2023
Title A Novel Method for Visualizing In-Vivo Rates of Protein Degradation Provides Insight into how TRIM28 Regulates Muscle Size
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Skeletal muscle size is controlled by the balance between protein synthesis and protein degradation. Given the essential role of skeletal muscle in maintaining a high quality of life, understanding the mechanisms that modulate this balance are of critical importance. Previously, we demonstrated that muscle-specific knockout of TRIM28 reduces muscle size and function, and in the current study, we discovered that this effect is associated with an increase in protein degradation and a dramatic reduction in the expression of Mettl21c. Importantly, we also determined that overexpression of Mettl21c is sufficient to induce hypertrophy in both control TRIM28 knockout muscles. Moreover, we developed a simple pulse-chase biorthogonal non-canonical amino acid tagging technique that enabled us to visualize the in-vivo rate of protein degradation, and with this technique were able to conclude that the hypertrophic effect of Mettl21c is due, at least in part, to an inhibition of protein degradation.
Overall design RNA-seq of mouse plantaris muscles. Four replicates each of:
Wild Type Sham
TRIM28 mKO Sham
Wild Type MTE
Contributor(s) Hornberger TA
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Submission date Jun 30, 2023
Last update date Jun 30, 2023
Contact name Troy A Hornberger
Organization name University of Wisconsin - Madison
Street address 2015 Linden Drive
City Madison
ZIP/Postal code 53706
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM7523793 TRIM28 mKO Sham1
GSM7523795 TRIM28 mKO Sham2
GSM7523796 Wild Type Sham1
BioProject PRJNA989589

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Supplementary file Size Download File type/resource
GSE236262_Processed_and_Raw_Read_RNA-sequencing_Data.xlsx 7.3 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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