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Series GSE236341 Query DataSets for GSE236341
Status Public on Mar 19, 2024
Title IL-33 controls IL-22-dependent antibacterial defense by modulating the microbiota [bulk RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary IL-22 plays a critical role in defending against mucosal infections, but how IL-22 production is regulated is incompletely understood. Here, we show that mice lacking IL-33 or its receptor ST2 (IL-1RL1) were more resistant to Streptococcus pneumoniae lung infection than wild-type animals, and that single nucleotide polymorphisms in IL33 and IL1RL1 were associated with pneumococcal pneumonia in humans. The effect of IL-33 on S. pneumoniae infection was mediated by negative regulation of IL-22 production by innate lymphoid cells (ILCs), but independent of ILC2s as well as IL-4 and IL-13 signaling. Moreover, IL-33´s influence on antibacterial defense was dependent on the housing conditions of the mice, and mediated by IL-33´s modulatory effect on the intestinal microbiota. Collectively, we reveal that IL-33 controls IL-22-dependent antibacterial defense by modulating the microbiota. Our study thus provides insight into the bidirectional crosstalk between the innate immune system and the microbiota and how it shapes susceptibility to bacterial infection.
 
Overall design Infection with S.pneumoniae, single cell digestion of lungs, sort for CD127+ ST2+ CD90+ ILC2s, bulkseq of 7 samples
 
Citation(s) 38781213
Submission date Jul 03, 2023
Last update date Jun 18, 2024
Contact name Bastian Opitz
Organization name Charité – Universitätsmedizin Berlin
Department Department of Infectious Diseases, Respiratory Medicine and Critical Care
Street address Augustenburgerplatz 1
City Berlin
ZIP/Postal code 13353
Country Germany
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (7)
GSM7528213 WT1 [2C]
GSM7528214 WT2 [3C]
GSM7528215 WT3 [5C]
This SubSeries is part of SuperSeries:
GSE236344 IL-33 controls IL-22-dependent antibacterial defense by modulating the microbiota.
Relations
BioProject PRJNA990664

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE236341_RAW.tar 23.6 Mb (http)(custom) TAR (of TXT)
GSE236341_make_table_featurecounts.R.gz 1.0 Kb (ftp)(http) R
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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