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Series GSE236628 Query DataSets for GSE236628
Status Public on Jan 22, 2024
Title The unique dual targeting of AGO1 by two types of PRMT enzymes promotes phasiRNA loading in Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Arginine / R methylation (R-met) of proteins is a widespread post-translational modification (PTM), deposited by a family of protein arginine / R methyl transferase enzymes (PRMT). Regulations by R-met are involved in key biological processes deeply studied in metazoan. Among those, post-transcriptional gene silencing (PTGS) can be regulated by R-met in animals and in plants. It mainly contributes to safeguard processes as protection of genome integrity in germ lines through the regulation of piRNA pathway in metazoan, or response to bacterial infection through the control of AGO2 in plants. So far, only PRMT5 has been identified as the AGO / PIWI R-met writer in higher eukaryotes. We uncovered that AGO1, the main PTGS effector regulating plant development, contains unique R-met features among the AGO / PIWI superfamily, and outstanding in eukaryotes. Indeed, AGO1 contains both symmetric (sDMA) and asymmetric (aDMA) R-dimethylations and is dually targeted by PRMT5 and by another type I PRMT in Arabidopsis thaliana . We showed also that loss of sDMA didn’t compromise AtAGO1 subcellular trafficking in planta. Interestingly, we underscored that AtPRMT5 specifically promotes the loading of a set of phasiRNA in AtAGO1. All our observations bring to consider this dual regulation of AtAGO1 in plant development and response to environment, and pinpoint the complexity of AGO1 post-translational regulation.
 
Overall design To investigate the impact of atprmt5 mutations on total sRNA accumulation and AtAGO1 sRNA loading in reproductive tissues. To do so, we carried out anti-AGO1 immunoprecipitations performed on close buds of A. thaliana, using Col0, atprmt5-2 and atprmt5-6 mutants. A fraction of inputs (total extracts) and all the IP products are used for sRNA extraction. The experiment includes biological duplicates.
 
Contributor(s) Barre-Villeneuve C, Carpentier M, Laudié M, Kuhne L, Lagrange T, Azevedo Favory J
Citation(s) 38321923
Submission date Jul 06, 2023
Last update date Apr 02, 2024
Contact name Jacinthe Azevedo Favory
E-mail(s) jacinthe.azevedo-favory@univ-perp.fr
Phone +33 4 30 19 81 22
Organization name CNRS
Lab LGDP
Street address 58 avenue Paul Alduy
City Perpignan
ZIP/Postal code 66860
Country France
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (48)
GSM7566746 wild type, buds, total sRNA fraction, replicate 1, 21 bp
GSM7566747 wild type, buds, total sRNA fraction, replicate 1, 22 bp
GSM7566748 wild type, buds, total sRNA fraction, replicate 1, 23 bp
Relations
BioProject PRJNA991926

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE236628_RAW.tar 800.0 Kb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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