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Series GSE237101 Query DataSets for GSE237101
Status Public on Oct 01, 2023
Title Bulk RNA-seq of mouse ileum of Lactobacillus rhamnosus GG or Ruminococcus gnavus monoassociated mice.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Lacticaseibacillus rhamnosus GG (LGG) is a widely consumed probiotic whose potential beneficial effects in humans have been examined in over 250 clinical trials. However, the mechanisms by which LGG modulates host gut physiology remains unknown. R. gnavus is a pathobiont that is strongly associated with inflammatory bowel diseases. Germ free mice were mono-associated with L. rhamonosus (LGG) or R. gnavus (RG) for 21 days, ileum bulk RNA-seq were performed to compare the transcriptomic profiles of LGG or RG associated mice with the transcriptome of germ-free mouse ileum.
Overall design Adult C57B/L6 germ-free mice were innoculated with LGG, RG or sterile PBS (as control). Successful colonization was confirmed by genomic PCR of fecal DNA using species specific primers. Twenty one days after inoculation, ilea were dissected from PBS (n=5), LGG (n=5) and RG (n=5) colonized mice. Bulk RNA-seq was performed to compare the transcriptomic profiles between germ-free (PBS) and LGG or RG colonized mice.
Contributor(s) Gao N, Flores J, Balasubramanian I, Bandyopadhyay S, Ferraris R, Suntornsaratoon P
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Submission date Jul 11, 2023
Last update date Oct 01, 2023
Contact name Nan Gao
Organization name Rutgers University
Street address 195 University Avenue
City Newark
State/province NJ
ZIP/Postal code 07102
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (15)
GSM7595061 PBS, mouse 1
GSM7595062 PBS, mouse 2
GSM7595063 PBS, mouse 3
BioProject PRJNA993709

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Supplementary file Size Download File type/resource
GSE237101_Processed_Data_File.xlsx 935.6 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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