 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 16, 2023 |
| Title |
scRNA of GBM tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Glioblastoma (GBM) presents a formidable clinical challenge due to its complex microenvironment. Here, we introduce tumor-associated foam cells (TAFs), a previously unidentified immune cell entity of lipid droplet (LD)-loaded macrophages, in GBM. Through extensive analyses of patient tumors, together with in vitro and in vivo investigations, we reveal that TAFs exhibit distinct pro-tumorigenic characteristics related to hypoxia, mesenchymal transition, angiogenesis, and impaired phagocytosis. Moreover, TAF presence correlates with worse patient outcome. Our mechanistic investigations demonstrate that TAF formation is facilitated by lipid cargo transfer from extracellular vesicles released by GBM cells. Importantly, we demonstrate that targeting key enzymes involved in LD formation, such as DGAT1 or ACSL, effectively disrupts TAF functionality. This study establishes TAFs as a prominent immune cell entity in GBM and provides valuable insights into their interplay within the microenvironment. Disrupting LD formation to target TAFs presents an interesting avenue for future therapeutic development in GBM.
|
| |
|
| Overall design |
scRNA-Seq
|
| |
|
| Contributor(s) |
Governa V, de Oliveira KG, Offer S, Magaña MC, Rudenstam AB, Li J, Beyer S, Johansson MC, Månsson A, Edvardsson C, Durmo F, Boukredine A, Gezelius E, Menard JA, Garza R, Jakobsson J, de Neergaard T, Sundgren PC, Nordenfelt P, Darabi A, Forsberg-Nilsson K, Talbot H, Bengzon J, Belting M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 18, 2023 |
| Last update date |
Aug 17, 2023 |
| Contact name |
Mattias Belting |
| E-mail(s) |
mattias.belting@med.lu.se
|
| Organization name |
Lund University
|
| Department |
Clinical Sciences Lund
|
| Lab |
Tumor Microenvironment
|
| Street address |
Barngatan
|
| City |
Lund |
| State/province |
Skåne |
| ZIP/Postal code |
SE-221 85 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA996138 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE237673_RAW.tar |
194.6 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...