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Series GSE237824 Query DataSets for GSE237824
Status Public on Dec 01, 2023
Title Mouse spleen LCM-ATACseq (B and T cell zones)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In situ transposition followed by laser capture microdissection was applied to spleen mouse tissue sections to demonstrate feasibility of LCM-based spatial chromatin accessibility analysis of regions of interest. B220 and CD3e immunostained B and T cell zones were analyzed.
Overall design Fresh frozen spleen tissue sections from C57BL/6J WT mice were tagmented in situ to assess chromatin accessibility. LCM was employed to collect a mini bulk of immunostained B and T cells. Two mini-bulk sizes were investigated: 100 single-captured nuclei or 10 larger captured-areas (patches) composed each of roughly 100 nuclei, for a total of ~1000 nuclei. Four technical replicates were collected at least per condition.
Contributor(s) Carraro C, Saglam A, Schultze JL, Beyer M
Citation(s) 37776856
Submission date Jul 20, 2023
Last update date Dec 01, 2023
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (18)
GSM7655014 B_patch_rep1 [Sample_24153]
GSM7655015 T_patch_rep1 [Sample_24154]
GSM7655016 B_patch_rep2 [Sample_24155]
This SubSeries is part of SuperSeries:
GSE237825 Mouse lung, brain and spleen LCM-ATACseq
BioProject PRJNA996891

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE237824_counts_GEO_revision.csv.gz 741.6 Kb (ftp)(http) CSV
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Processed data are available on Series record

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