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| Status |
Public on Jul 25, 2023 |
| Title |
Flexible and scalable control of T cell memory by a reversible epigenetic switch (sci-fate-seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we use a minimal ex vivo culture system to track CD8 T cell differentiation at the single cell and clonal lineage level during activation. To characterize early heterogeneity in memory and effector differentiation in this system, we subjected cells to temporally-resolved single-cell transcriptomic profiling using sci-fate-seq (Cao et al., Nature Biotech, 202).
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| Overall design |
Cells were activated ex vivo and subjected at days 1, 2, and 4 to 4-thiouridine (4sU) pulse-labeling for 2 hrs, enabling detection of newly synthesized mRNA at each time point.
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| Contributor(s) |
Abadie K, Yang W, Cao J, Shendure J, Kueh H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 20, 2023 |
| Last update date |
Jul 25, 2023 |
| Contact name |
Kathleen Abadie |
| E-mail(s) |
abadiek@uw.edu
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| Organization name |
University of Washington
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| Department |
Bioengineering
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| Lab |
Kueh Lab
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| Street address |
3720 15th Ave NE
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| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE237830 |
Flexible and scalable control of T cell memory by a reversible epigenetic switch |
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| Relations |
| BioProject |
PRJNA996895 |
