GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE23792 Query DataSets for GSE23792
Status Public on Aug 25, 2010
Title Genome-wide analysis of insulin-like growth factor 1 receptor (IGF1R) binding sites in DFB cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The insulin-like growth factor 1 receptor (IGF-1R) plays crucial roles in developmental and cancer biology. Most of its biological effects have been ascribed to its tyrosine kinase activity. We report that IGF-1 promotes the modification of IGF-1R by small ubiquitin-like modifier protein-1 (SUMO-1) and its translocation to the nucleus. Nuclear IGF-1R associated with enhancer-like elements and increased transcription in reporter assays. We used ChIP-seq to examine the interaction of IGF-1R with DNA on a genome-wide scale. Analysis of the data set resulted in 568 candidate peaks, that is, statistically significant IGF-1R-enriched regions. The IGF-1R-enriched regions were divided into five classes on the basis of their location relative to known genes. Most of the IGF-1R-interacting sites (80%) were located distal from any annotated gene (intergenic), 6.3% were located in introns, 6.3% in exons, 3.4% were <20 kb upstream of an annotated transcript start site (5'UTR + 20 kb upstream), and 3.6% were <20 kb downstream of an annotated transcript end site (3'UTR + 20 kb downstream).
Overall design Analysis of the genomic interaction of IGF1R in DFB cells
Contributor(s) Sehat B, Tofigh A, Lin Y, Trocmé E, Liljedahl U, Lagergren J, Larsson O
Citation(s) 20145208
Submission date Aug 25, 2010
Last update date May 15, 2019
Contact name Ali Tofigh
Organization name McGill University
Street address McGill Centre for Bioinformatics , Life Sciences Complex, Bellini Building, room 435, 3649 Promenade Sir-William-Osler
City Montreal
State/province Quebec
ZIP/Postal code H3G 0B1
Country Canada
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (1)
GSM586973 IGF1R_CHiPseq
SRA SRP003362
BioProject PRJNA130715

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23792_RAW.tar 26.1 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap