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Series GSE237939 Query DataSets for GSE237939
Status Public on May 31, 2024
Title N-terminal acetylation of the Set1-COMPASS fine-tunes H3K4 methylation patterns (ChIPseq for shg1 and spp1 deletion strains)
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary H3K4 methylation is a conserved histone modification crucial for gene regulation, yet the post-translational modifications of the Set1-COMPASS complex remain largely unexplored. This study elucidates the significance of N-terminal acetylation in modulating H3K4 methylation patterns. Firstly, loss of NatA complex resulted in a significant decrease in H3K4me3 levels and a shift of H3K4me2 from 5' transcribed regions to promoters. Importantly, NatA physically interacted with the Set1-COMPASS complex and facilitated N-terminal acetylation of the Shg1 subunit. Surprisingly, deletion of SHG1 or mutation of the acetylation site (A2P) in Shg1 led to reduced H3K4 methylation in cells lacking Spp1. Additionally, NatB complex also contributed to H3K4 methylation regulation, potentially through N-terminal acetylation of Swd1. These findings highlight the novel role of N-terminal acetylation in fine-tuning the function of the Set1-COMPASS complex and shaping H3K4 methylation patterns. This study sheds light on the intricate regulation of H3K4 methylation and emphasizes the significance of N-terminal acetylation as a regulatory mechanism in this process.
Overall design Chromatin immunoprecipitation DNA sequencing (ChIP-seq) for histone modifications H3K4me2 and H3K4me3 as well as histone H3 in WT, shg1Δ, spp1Δ and shg1Δspp1Δ strains in Saccharomyces cerevisiae.
Contributor(s) Woo H, Oh J, Lee J, Kim T
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Submission date Jul 21, 2023
Last update date Jun 01, 2024
Contact name Junsoo Oh
Organization name Kangwon National University
Street address Kangwondaehakgil-1
City Chuncheon
ZIP/Postal code 24341
Country South Korea
Platforms (1)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
Samples (24)
GSM7656706 YF336-1-H3K4me3
GSM7656707 YF336-2-H3K4me3
GSM7656708 YF336-1-H3K4me2
This SubSeries is part of SuperSeries:
GSE238070 N-terminal acetylation of the Set1-COMPASS fine-tunes H3K4 methylation patterns
BioProject PRJNA997269

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Supplementary file Size Download File type/resource
GSE237939_RAW.tar 777.5 Mb (http)(custom) TAR (of BW) 80.2 Mb (ftp)(http) BW 79.9 Mb (ftp)(http) BW 76.0 Mb (ftp)(http) BW 80.6 Mb (ftp)(http) BW 79.9 Mb (ftp)(http) BW 76.2 Mb (ftp)(http) BW 81.3 Mb (ftp)(http) BW 74.7 Mb (ftp)(http) BW 65.9 Mb (ftp)(http) BW 82.3 Mb (ftp)(http) BW 71.2 Mb (ftp)(http) BW 39.0 Mb (ftp)(http) BW
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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