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Series GSE239355 Query DataSets for GSE239355
Status Public on Mar 19, 2024
Title Fetal Sex Does Not Influence Rates of Gastrulation-Stage Alcohol-Induced Craniofacial Malformations in C57BL/6J Mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Prenatal alcohol exposure during mouse gastrulation (embryonic day [E] 7 in mice, corresponding to ~3rd week of human pregnancy) impairs eye, facial, and cortical development, recapitulating birth defects characteristic of Fetal Alcohol Syndrome (FAS). However, the impact of sex on the prevalence or severity of craniofacial features associated with FAS is currently unknown. The current study administered either alcohol (2.9g/kg, two i.p. doses, four hours apart) or vehicle control to pregnant C57BL/6J females on E7 and assessed fetal morphology at E17. Whereas sex did not affect fetal size in vehicle-treated litters, alcohol-exposed females were smaller than both control females and alcohol-treated males. Alcohol exposure increased the incidence of eye defects to a similar degree in males and females. Together, this suggests that females might be more sensitive to the general developmental effects of alcohol, but not to the effects of alcohol specifically on the craniofacies. We also established a developmental transcriptional baseline via whole transcriptomic analysis of untreated E7 embryos, and found 214 differentially expressed genes in females vs. males, including those in pathways related to cilia and mitochondria structure and function, histone demethylase activity, and pluripotency.
Overall design RNA-seq between embryonic day 7.0 (E7.0) male and female embryos in C57BL/6J mice
Contributor(s) Boschen KE, Dragicevich CJ, Fish EW, Hepperla AJ, Simon JM, Parnell SE
Citation(s) 38116840
Submission date Jul 26, 2023
Last update date Mar 19, 2024
Contact name Austin J Hepperla
Organization name University of North Carolina at Chapel Hill
Department Genetics
Street address 7018B Mary Ellen Jones Building
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM7663570 E7_embryo_male_WT_rep1
GSM7663571 E7_embryo_male_WT_rep2
GSM7663572 E7_embryo_male_WT_rep3
BioProject PRJNA998893

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Supplementary file Size Download File type/resource
GSE239355_Parnell_E7_FemaleVsMale_DESeq2_results.xlsx 7.1 Mb (ftp)(http) XLSX
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