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| Status |
Public on Aug 02, 2023 |
| Title |
Identification of genes associated with the development of silk glands using transcriptomics in silkworm (Bombyx mori) |
| Organism |
Bombyx mori |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The silk gland development has a greater impact on silk yields in silkworms. Silk glands from three pure silkworm strains (A798, A306, and XH) with different silk gland weight phenotypes were compared using transcriptome, proteomics, and WGCNA. Five genes (BGIBMGA002524, BGIBMGA002629, BGIBMGA005659, BGIBMGA005711, and BGIBMGA010889) may be strongly associated with the growth of silk glands to be confirmed. These DEGs encoded alkylglycerol monooxygenase (AGMO), glucose dehydrogenase (GDH), zonadhesin (ZAN), odorant binding protein (OBPs), and β-fructofuranosidase (INV), respectively. PCR and ELISA were used to verify the mRNA and protein expression of five genes in the silk glands and tissues of 18 silkworm strains. The GO results showed that four genes have higher levels of expression and participate in glycogen metabolism, fatty acid synthesis, and branched-chain amino acid metabolism, thus, promoting growth and silk proteins synthesis.
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| Overall design |
We selected three pure silkworm strains (A798, A306, and XH) with different silk gland weight phenotypes to investigate the silkworm's development-related genes. Then, we carried out the gene expression levels using the data obtained from the RNA-seq of three pure silkworm strains. Comparative gene expression profiling analysis of RNA-seq data for A306 against A798 and XH against A798.
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| Contributor(s) |
Sun L, Sun B, Chen L, Ge Q, Chen K |
| Citation missing |
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| Submission date |
Jul 26, 2023 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Sun Lindan |
| E-mail(s) |
sunlindan@ujs.edu.cn
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| Phone |
13675118671
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| Organization name |
Jiangsu University
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| Department |
School of Life and Sciences
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| Street address |
301 xuefu road
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| City |
Zhenjiang |
| ZIP/Postal code |
212013 |
| Country |
China |
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| Platforms (1) |
| GPL23127 |
Illumina HiSeq 2500 (Bombyx mori) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA999012 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE239380_genes_fpkm_expression.txt.gz |
337.8 Kb |
(ftp)(http) |
TXT |
| GSE239380_raw_count.txt.gz |
1.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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