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Series GSE239741 Query DataSets for GSE239741
Status Public on Aug 01, 2023
Title A somatic cell cloned rhesus monkey obtained by trophoblast replacement [RNA-seq]
Organism Macaca fascicularis
Experiment type Expression profiling by high throughput sequencing
Summary Somatic cell nuclear transfer (SCNT) has been used to clone cynomolgus monkeys (Macaca fascicularis), but the cloning of other non-human primate species remains to be achieved. On the other hand, our histological examination indicated severe calcification of the placenta of SCNT fetuses. Additionally, we found that some of the maternal-biased imprinting genes were aberrantly lost in the cloned embryos by multi-omics analysis. We thus developed a trophoblast replacement (TR) method, providing an ICSI-derived placenta for the SCNT fetus, to support the full-term development of the cloned embryos after implantation. By combining this TR method with treatment with epigenetic modification factors, we obtained a healthy somatic cell cloned rhesus monkey. Thus, TR represents a useful approach for rhesus monkey cloning.
 
Overall design RNA sequencing of the trophoblast cells of monkey ICSI and SCNT embryos
 
Contributor(s) Liao Z
Citation(s) 38228612
Submission date Aug 01, 2023
Last update date Jan 29, 2024
Contact name Liao Zhaodi
E-mail(s) zhaodiliao@ion.ac.cn
Phone 17521632708
Organization name Chinese Academy of Sciences
Department Institute of Neuroscience
Street address 500, Qiangye Road
City Shanghai
ZIP/Postal code 2016000
Country China
 
Platforms (1)
GPL28212 Illumina NovaSeq 6000 (Macaca fascicularis)
Samples (4)
GSM7670817 ICSI-TE-RNAseq-rep1
GSM7670818 ICSI-TE-RNAseq-rep2
GSM7670819 SCNT-TE-RNAseq-rep1
This SubSeries is part of SuperSeries:
GSE239746 A somatic cell cloned rhesus monkey obtained by trophoblast replacement
Relations
BioProject PRJNA1000796

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE239741_trophoblast_counts.txt.gz 2.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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