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| Status |
Public on May 19, 2011 |
| Title |
The Cohesin Complex Cooperates with Pluripotency Transcription Factors in the Maintenance of Embryonic Stem Cell Identity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Embryonic stem cells (ESCs) cells run a self-renewal gene expression program, requiring the expression of certain transcription factors accompanied by a particular chromosome organization to maintain a balance between pluripotency and the capacity for rapid differentiation. However, how transcriptional regulation is linked to chromosome organization in ESCs remains enigmatic. Here we show that Cohesin exhibits a functional role in maintaining ESC identity through association with the pluripotency transcriptional network. ChIP-seq analyses of the cohesin subunit Rad21 reveal an ESC specific cohesin binding pattern that is characterized by a CTCF independent colocalization of cohesin with pluripotency related transcription factors. Upon ESC differentiation, these binding sites disappear and instead new CTCF independent Rad21 binding sites emerge, which are enriched for binding sites of transcription factors implicated in early differentiation. Furthermore, knock-down of cohesin subunits causes expression changes that are reminiscent of the depletion of key pluripotency transcription factors, demonstrating the functional relevance of the cohesin - pluripotency transcriptional network association. Finally, we show that Nanog physically interacts with the cohesin interacting proteins Stag1 and Wapl, further substantiating this association. Based on these findings we propose that a dynamic placement of cohesin by pluripotency transcription factors contributes to a chromosome organization supporting the ESC expression program.
This SuperSeries is composed of the SubSeries listed below.
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| Overall design |
Refer to individual Series
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| |
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| Contributor(s) |
Nitzsche A, Paszkowski-Rogacz M |
| Citation(s) |
21589869 |
| |
| Submission date |
Sep 08, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Maciej Paszkowski-Rogacz |
| Organization name |
Technische Universität Dresden
|
| Department |
UCC / Medical Faculty and University Hospital Carl Gustav Carus
|
| Lab |
Medical Systems Biology (Prof. Buchholz)
|
| Street address |
Tatzberg 47/49
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (14)
|
| GSM589801 |
Luc control esiRNA, biological rep1 |
| GSM589802 |
Luc control esiRNA, biological rep2 |
| GSM589803 |
Luc control esiRNA, biological rep3 |
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| This SuperSeries is composed of the following SubSeries: |
| GSE23923 |
Expression data from Rad21 knock-down in ES cells |
| GSE24029 |
Genome-wide maps of Rad21 in R1/E embryonic stem cells and R1/E derived Embryoid bodies |
| GSE25777 |
Genome-wide maps of CTCF in R1/E embryonic stem cells |
|
| Relations |
| BioProject |
PRJNA130275 |
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