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Series GSE240464 Query DataSets for GSE240464
Status Public on May 24, 2024
Title Enhanced Phase Separation Mediated Oncogenicity [ChIP-Seq, 2]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromosome translocation is known to generate oncogenes such as SS18-SSX1, but the underlying mechanism remains unknown. Here we show that enhanced phase separation acquired by translocation mediates oncogenic transformation. SSX fusion dramatically enhances the phase separation property of SS18 protein through H2AK119ub. Super condensates generated by SS18-SSX recruit acetyltransferases CBP/P300 as wild type SS18, but exclude HDACs such that H3K27ac accumulates abnormally at loci from those normally occupied by SS18, resulting aberrant silencing and activation of target genes. Consistently, we show that inhibition of H3K27ac can attenuate the tumorigenicity of the fusion oncoprotein. These results provide the first case for phase separation as a transforming event to generate oncogene and super phase separation can be targeted for cancer therapy.
Overall design We analyzed the endogenous SS18 protein cupancy during synovial sarcomagenesis triggered by SS18-SSX1 overexpression and the location of LLPS deficiency mutant SS(YA).
Contributor(s) Kuang J, Pei D
Citation(s) 38678233
Submission date Aug 09, 2023
Last update date May 24, 2024
Contact name kuang junqi
Organization name westlake University
Street address No.18 Shilongshan Road
City hangzhou
ZIP/Postal code 310024
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM7698397 EGFP-endo_SS18
GSM7698398 SS-endo_SS18
GSM7698399 SS-HA
This SubSeries is part of SuperSeries:
GSE211456 Enhanced Phase Separation Mediated Oncogenicity
BioProject PRJNA1003848

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Supplementary file Size Download File type/resource
GSE240464_RAW.tar 554.6 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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