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Series GSE240522 Query DataSets for GSE240522
Status Public on Dec 01, 2023
Title The epigenetic landscape of oligodendrocyte progenitors changes with time
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Adult oligodendrocyte progenitors (aOPCs) share with their neonatal counterpart (nOPCs) the ability to give rise to myelinating oligodendrocytes, but they also display unique functional features. This study addresses the molecular mechanisms underlying the intrinsic differences between these two populations. Using RNA-sequencing and unbiased histone proteomics analysis, we define the unique transcriptome and histone marks of aOPCs. We describe the lower proliferative capacity and higher levels of expression of oligodendrocyte specific genes in aOPC compared to nOPC. We also identify greater levels of activating H4K8ac histone marks in aOPCs compared to nOPCs, and increased occupancy of this mark at chromatin locations corresponding to oligodendrocyte-specific transcription factors and lipid metabolism genes. Pharmacological inhibition of H4K8ac deposition reduces the levels of these transcripts in aOPCs, rendering their transcriptome more similar to nOPCs. The repressive H4K20me3 mark is also higher in aOPCs compared to nOPCs and pharmacological inhibition of its deposition results in increased levels of genes related to the mature oligodendrocyte state. Overall, this study identifies two histone marks which are important for the unique transcriptional identity of aOPCs.
 
Overall design Chromatin immunoprecipitaion with H4K8ac antibody followed by sequencing (ChIP-seq) on sorted neonatal oligodendrocyte progenitors (nOPCs) and adult OPCs (aOPCs) from PdgfrαH2BEGFP mice.
 
Contributor(s) Casaccia P, Dansu DK, Huang D, Li M, Sauma S, Moyon S
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Submission date Aug 10, 2023
Last update date Dec 01, 2023
Contact name Patrizia Casaccia
E-mail(s) pcasaccia@gc.cuny.edu
Organization name ASRC-CUNY
Department Neuroscience
Street address 85 St Nicholas Terrace
City New York
State/province NY
ZIP/Postal code 10031
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (12)
GSM7699378 Neonatal oligodendrocyte progenitor cell biol rep 1 (P5)
GSM7699379 Neonatal oligodendrocyte progenitor cell input biol rep 1 (P5)
GSM7699380 Neonatal oligodendrocyte progenitor cell biol rep 2 (P5)
Relations
BioProject PRJNA1004178

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE240522_H4K8Ac_diffBind.narrowPeak.gz 1.5 Mb (ftp)(http) NARROWPEAK
GSE240522_RAW.tar 2.0 Gb (http)(custom) TAR (of BW, TXT)
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Raw data are available in SRA

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