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| Status |
Public on Aug 10, 2024 |
| Title |
Single-cell time-resolved multi-omics reveal apoptotic and ferroptotic heterogeneity during foam cell formation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophage-derived foam cell plays a pivotal role in the plaque formation and rupture during the progression of atherosclerosis. Foam cells are destined to divergent cell fate and functions in response to external stimuli based on their internal states, which however is hidden in the traditional studies based on population of cells. Herein, we used time-resolved and single-cell multi-omics to investigate the macrophage heterogeneity along foam cell formation. Dynamic metabolome and lipidome outlined the dual regulating axis of inflammation and ferroptosis. Single cell metabolomics and lipidomics further demonstrated a macrophage continuum featuring a differed susceptibility to apoptosis and ferroptosis. Using single-cell transcriptomic profiling, we verified the divergent cell fate toward apoptosis or ferroptosis. Therefore, the molecular choreography underlying the divergent cell fate during foam cell formation was revealed, which is of high significance for the understanding of the pathogenesis of atherosclerosis and development of new drug targets.
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| Overall design |
One PBMC-derived late-stage foam cell.
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| Contributor(s) |
Wang Y, Lin L, Qiao L |
| Citation missing |
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| Submission date |
Aug 14, 2023 |
| Last update date |
Aug 10, 2024 |
| Contact name |
Yiwen Wang |
| E-mail(s) |
16307110373@fudan.edu.cn
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| Organization name |
Fudan University
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| Street address |
2005, Songhu Road, Yangpu District
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| City |
Shanghai |
| ZIP/Postal code |
200438 |
| Country |
China |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA1005309 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE240820_RAW.tar |
190.9 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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