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Series GSE241026 Query DataSets for GSE241026
Status Public on Jun 05, 2024
Title TCF7L1 regulates colorectal cancer cell migration and invasion by repressing GAS1 expression (ChIP-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Dysregulation of the canonical Wnt/β-catenin signaling pathway is a hallmark of colorectal cancer (CRC). The T-cell factor/lymphoid enhancer factor (TCF/LEF; hereafter, TCF) family of transcription factors are critical regulators of canonical Wnt/β-catenin target gene expression. Despite similarity across DNA-binding and protein-binding domains, TCF family members differentially regulate target gene expression in CRC. Of the four TCF family members, TCF7L1 predominantly functions as a transcriptional repressor and it has an oncogenic role in CRC. Despite this role, few target genes regulated by TCF7L1 in CRC have been identified. Through RNA-sequencing of transcripts differentially expressed in control and TCF7L1-overexpressing HCT116 cells, we identified 397 genes that were repressed directly, or indirectly, by TCF7L1. Gene set enrichment analysis found genes associated with epithelial-mesenchymal transition amongst those that are differentially expressed. By silencing and overexpressing TCF7L1 in CRC cell lines, we found that TCF7L1 promoted migration, invasion, and adhesion in vitro. Chromatin immunoprecipitation followed by sequencing (ChIP-sequencing) localized 3661 TCF7L1 binding sites within the CRC genome. Comparison of genes whose expression was downregulated by TCF7L1 and genes with TCF7L1 binding sites revealed 41 novel targets directly regulated by TCF7L1. Among these, we localized a TCF7L1 promoter-proximal binding site within the growth arrest specific 1 (GAS1) gene. We found that ectopic GAS1 expression rescued the TCF7L1-mediated increase in migration and invasion in CRC cells. These findings uncover a novel role for TCF7L1 in repressing GAS1 expression to promote migration and invasion of CRC cells in vitro.
 
Overall design Chromatin immunoprecipitation (ChIP) sequencing for DNA fragments immunoprecipitated with an anti-FLAG antibody for FLAG-tagged TCF7L1 in Dox-treated TCF7L1-depleted HCT116 cells expressing exogenous TCF7L1 or TCF7L1-MUT (DNA binding domain mutant)
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***Authors state the following regarding their missing raw data:
The library was constructed and processed by Novogene Co., Ltd in 2021. However, we currently only have the BED, bigWig, and narrowPeaks file formats.
 
Contributor(s) King C, Eshelman M, Yochum G
Citation(s) 38816533
Submission date Aug 16, 2023
Last update date Jun 06, 2024
Contact name Gregory S Yochum
Organization name The Pennsylvania State University, College of Medicine
Street address 700 HMC Cres Rd
City Hershey
State/province PA
ZIP/Postal code 17033
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2)
GSM7714040 HCT116, shTCF7L1-2, Dox-treated to express pCW57.1-TCF7L1 ChIP-Seq
GSM7714041 HCT116, shTCF7L1-2, Dox-treated to express pCW57.1-TCF7L1-MUT ChIP-Seq
This SubSeries is part of SuperSeries:
GSE241028 TCF7L1 regulates colorectal cancer cell migration and invasion by repressing GAS1 expression
Relations
BioProject PRJNA1006070

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241026_RAW.tar 247.2 Mb (http)(custom) TAR (of BED, BW, NARROWPEAK)
Raw data not provided for this record
Processed data provided as supplementary file

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