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Series GSE241364 Query DataSets for GSE241364
Status Public on May 30, 2024
Title Oncogenic Calreticulin Induces Immune Escape by Stimulating TGF-β Expression and Regulatory T Cell Expansion in the Bone Marrow Microenvironment - scRNAseq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We established a model for myeloproliferative disease in mice, that is based on primary bone marrow expressing human Thrombopoietin receptor and human mutant Calrdel52. To learn more about cellular and transcriptional rearrangements in the bone marrow we performed single cell RNA sequencing comparing bone marrow cells of empty vector control mice with mice that received MPL-Calrdel52 bone marrow.
 
Overall design A total of two mice for each group were analyzed. Two Mice received empty vector control BM. Two mice received MPL-Calr(del52) BM. Vector included an IRES-GFP sequence as transduction/engraftment control. Engraftment and disease phenotype was checked before analysis.
 
Contributor(s) Andrieux G, Boerries M, Schmidt D, Zeiser R
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 21, 2023
Last update date May 31, 2024
Contact name Geoffroy Andrieux
Organization name University clinics Freiburg
Street address Breisacherstr 153
City Freiburg
ZIP/Postal code 79110
Country Germany
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM7727712 Empty vector Bone Marrow 2
GSM7727713 Empty vector Bone Marrow 3
GSM7727714 MPL-Calr(del52) Bone Marrow 3
This SubSeries is part of SuperSeries:
GSE241365 Oncogenic Calreticulin Induces Immune Escape by Stimulating TGF-β Expression and Regulatory T Cell Expansion in the Bone Marrow Microenvironment
Relations
BioProject PRJNA1007754

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241364_RAW.tar 197.6 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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