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| Status |
Public on Nov 27, 2023 |
| Title |
KEAP1 mutation in lung adenocarcinoma promotes immune evasion and immunotherapy resistance [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Immune cells were isolated from the lungs of mice bearing KEAP1 mutant or wildtype tumors. We found that in KEAP1 mutant tumors there is a reduction in CD103 DCs and early activated T cells as well as an increase in exhausted CD8 T cells in comparison to WT controls.
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| Overall design |
KEAP1 mutant (n=1) or wildtype (n=1) murine KP lung cancer cells were injected IV into C57BL/6 mice. Lungs were harvested and extravascular CD45 cells were sorted for scRNA-seq. Samples were stained with hashtag antibodies. Libraries were generated for gene expression, hashtags, and TCR sequencing and were then sequenced.
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| Contributor(s) |
Zavitsanou AM, Pillai R, Hao Y, Tsirigos A, Koralov S, Papagiannakopoulos T |
| Citation(s) |
37889752 |
| |
| Submission date |
Aug 22, 2023 |
| Last update date |
Nov 27, 2023 |
| Contact name |
Yuan Hao |
| Organization name |
NYU Langone Health
|
| Street address |
Laura and Isaac Perlmutter Cancer Center
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| City |
New York |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE241482 |
KEAP1 mutation in lung adenocarcinoma promotes immune evasion and immunotherapy resistance. |
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| Relations |
| BioProject |
PRJNA1008265 |
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