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Series GSE241571 Query DataSets for GSE241571
Status Public on Oct 02, 2023
Title Cryptosporidium infection of human small intestinal epithelial cells induces type III interferon expression and impairs the infectivity of live-attenuated rotavirus vaccines [hALI_Cp]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We developed a stem cell-derived culture system for C. hominis using human enterocytes differentiated under air-liquid interface (ALI) conditions. Human ALI (hALI) cultures supported robust growth and complete development of C. hominis in vitro including all life cycle stages.
C. hominis infection induced a strong interferon response from enterocytes, likely driven by an endogenous dsRNA virus in the parasite. Prior infection with Cryptosporidium induced type III IFN secretion and consequently blunted infection with Rotavirus, suggesting such co-infections may alter vaccine efficacy.
 
Overall design Comparative gene expression profiling analysis of RNA-seq data for hALI infected with C. parvum for 24h, or without infection.
 
Contributor(s) Greigert V, Saraav I, Sibley LD
Citation(s) 37693422, 38189373
Submission date Aug 23, 2023
Last update date Jan 29, 2024
Contact name L. David Sibley
E-mail(s) sibley@wustl.edu
Organization name Washington University School of Medicine
Department Molecular Microbiology
Street address 425 S. Euclid Ave
City St. Louis
State/province MO
ZIP/Postal code 63110-1093
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM7731118 Non-infected cells, replicate 1 [ni_24_R1_T1]
GSM7731119 Non-infected cells, replicate 2 [ni_24_R2_T1]
GSM7731120 Non-infected cells, replicate 3 [ni_24_R3_T1]
This SubSeries is part of SuperSeries:
GSE241572 Cryptosporidium infection of human small intestinal epithelial cells induces type III interferon expression and impairs the infectivity of live-attenuated rotavirus vaccines.
Relations
BioProject PRJNA1008720

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241571_normalized_filtered_data.csv.gz 293.5 Kb (ftp)(http) CSV
GSE241571_raw_counts.csv.gz 1.1 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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