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Series GSE241681 Query DataSets for GSE241681
Status Public on Oct 03, 2023
Title The Tbx20-TLE Interaction is Essential for the Maintenance of the Second Heart Field
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary T-box transcription factor 20 (Tbx20) plays a multifaceted role in cardiac morphogenesis and controls a broad gene regulatory network. However, the mechanism by which Tbx20 activates and represses target genes in a tissue-specific and temporal manner remains unclear. Studies show that Tbx20 directly interacts with the Transducin-like Enhancer of Split (TLE) family of proteins to mediate transcriptional repression. However, a function for the Tbx20-TLE transcriptional repression complex during heart development has yet to be established. We created a mouse model with a two-amino acid substitution in the Tbx20 EH1 domain, thereby disrupting the Tbx20-TLE interaction. Disruption of this interaction impaired critical morphogenic events, including cardiac looping and chamber formation. Transcriptional profiling of Tbx20EH1Mut hearts and analysis of putative direct targets revealed misexpression of the retinoic acid pathway and cardiac progenitor genes. Further, we show that altered cardiac progenitor development and function contribute to the severe cardiac defects in our model. Our studies indicate that TLE-mediated repression is a primary mechanism by which Tbx20 controls gene expression. 
Overall design To investigate the role of the Tbx20/TLE repressive complex in heart development, we generated a mouse model with a two amino acid substitution in the Tbx20 engrailed homology domain (Tbx20EH1Mut). We then performed RNA-seq analysis on wild-type and Tbx20EH1Mut hearts at embryonic day 9.5.
Contributor(s) Edwards W, Conlon F
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Submission date Aug 24, 2023
Last update date Oct 04, 2023
Contact name Whitney Edwards
Phone 5129975191
Organization name University of North Carolina Chapel Hill
Department Biology
Lab 220 Fordham Hall
Street address 133 North Medical Drive
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM7733686 WT-1
GSM7733687 WT-2
GSM7733688 WT-3
BioProject PRJNA1009104

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241681_RAW.tar 26.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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