NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE242143 Query DataSets for GSE242143
Status Public on Mar 26, 2024
Title DNA methylation status determines the sensitivity of repeats to restriction by the HUSH-MORC2 complex
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary The human silencing hub (HUSH) complex binds to transcripts of LINE-1 retrotransposons (L1s) and other genomic repeats, recruiting MORC2 and other effectors to remodel chromatin. However, how HUSH and MORC2 operate alongside DNA methylation, a central epigenetic regulator of repeat transcription, remains poorly understood. Here we interrogate this relationship in human neural progenitor cells (hNPCs), a somatic model of brain development that tolerates removal of DNA methyltransferase DNMT1. Upon loss of MORC2 or HUSH subunit TASOR in hNPCs, L1s remain silenced by robust promoter methylation. However, genome demethylation and activation of evolutionarily-young L1s attracts MORC2 binding. Simultaneous depletion of DNMT1 and MORC2 causes massive accumulation of L1 transcripts. We identify the same mechanistic hierarchy at pericentromeric ╬▒-satellites and clustered protocadherin genes, repetitive elements important for chromosome structure and neurodevelopment respectively. Our data delineate the independent epigenetic control of repeats in somatic cells, with implications for understanding the vital functions of HUSH-MORC2 in hypomethylated contexts throughout human development.
 
Overall design Refer to individual Series (listed below).
 
Contributor(s) Pandiloski N
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Sep 01, 2023
Last update date Mar 27, 2024
Contact name Ninoslav Pandiloski
E-mail(s) ninoslav.pandiloski@med.lu.se
Organization name Lund University
Street address S├Âlvegatan 17
City Lund
ZIP/Postal code 223 62
Country Sweden
 
Platforms (3)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL26167 PromethION (Homo sapiens)
Samples (75)
GSM7697250 CR117_LV3599_Control_IgG_rep1
GSM7697251 CR117_LV3599_Control_IgG_rep2
GSM7697252 CR112_LV3599_Control_IgG_rep3
This SuperSeries is composed of the following SubSeries:
GSE240384 DNA methylation status determines the sensitivity of repeats to restriction by the HUSH-MORC2 complex [CUT&RUN]
GSE240386 DNA methylation status determines the sensitivity of repeats to restriction by the HUSH-MORC2 complex [Bulk RNA-seq]
GSE262482 DNA methylation status determines the sensitivity of repeats to restriction by the HUSH-MORC2 complex [ONT whole genome DNA]
Relations
BioProject PRJNA1011851

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE242143_RAW.tar 23.0 Gb (http)(custom) TAR (of BW, TSV)
SRA Run SelectorHelp

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap