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Series GSE242203 Query DataSets for GSE242203
Status Public on Sep 06, 2023
Title Mechanism of Hyperproteinemia-Induced Damage to Female Reproduction in a Genetic Silkworm Model
Organism Bombyx mori
Experiment type Expression profiling by high throughput sequencing
Summary Hyperproteinemia is a metabolic disorder characterized by abnormally elevated plasma protein concentrations (PPC) in humans and animals. Here, a genetic silkworm model with high PPC was employed to investigate the effect of elevated PPC on female reproduction. Transcriptomic analysis revealed that high PPC induces downregulation of the ovarian development-related genes and disrupts ovarian sugar metabolism. Biochemical and endocrinal analyses revealed that high PPC increases trehalose and glucose levels in hemolymph and glycogen content in the fat body through activation of the gluconeogenic pathway and inhibition of the IIS-AKT pathway, thus disrupting characteristic metabolic homeostasis of sugar in the ovary. These resulted in ovarian developmental delay as well as reduced number and poor quality of eggs. Insulin supplementation effectively increased egg numbers by lowering blood sugar. These collective results provide new insights into the mechanisms by which high PPC negatively affects female reproduction and support the potential therapeutic effects of insulin.
Overall design To identify the molecular targets of high PPC that affect female reproduction, RNA-seq analysis was performed on ovarian tissues at the P2 and P4 stages
Contributor(s) Jiang G, Wang G, Luo C, Wang Y, Qiu J, Peng R, Sima Y, Xu S
Citation(s) 37752953
Submission date Sep 01, 2023
Last update date Oct 03, 2023
Contact name Guihua Jiang
Phone +8651265880185
Organization name Soochow University
Street address Renai Road 199, Dushu Lake Higher Education Town
City Suzhou
ZIP/Postal code 215031
Country China
Platforms (1)
GPL29210 Illumina NovaSeq 6000 (Bombyx mori)
Samples (12)
GSM7754973 WT-P2-1
GSM7754974 WT-P2-2
GSM7754975 WT-P2-3
BioProject PRJNA1011975

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GSE242203_Processed_data_files.txt.gz 426.2 Kb (ftp)(http) TXT
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