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Series GSE242404 Query DataSets for GSE242404
Status Public on Dec 01, 2023
Title snRNA-seq of aldosterone producing adenoma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Recent advances in omics techniques have allowed detailed genetic characterization of aldosterone-producing adenoma (APA). The pathogenesis of APA is characterized by tumorigenesis-associated aldosterone synthesis. The pathophysiological intricacies of APAs have not yet been elucidated at the level of individual cells . Therefore, a single-cell level analysis is speculated to be valuable in studying the differentiation process of APA. We conducted single-nuclei RNA sequencing (snRNA-seq) of APAs and non-functional adenomas (NFA) obtained from three and two patients, respectively.The snRNA-seq revealed the intratumoral heterogeneity of APA and identified cell populations consisting of a shared cluster of NFA and APA. In addition, we extracted two cell differentiation pathways in APA and obtained a cell population specialized in aldosterone synthesis. Genes related to ribosomes and neurodegenerative diseases were upregulated in one of these pathways, whereas those related to the regulation of actin cytoskeleton were upregulated in the other pathway. Furthermore, the total RNA reads in the nucleus were higher in highly differentiated clusters, indicating a marked activation of transcription per cell. The snRNA-seq provides novel differentiation pathways within APA tumors, which will further support our understanding of its pathophysiology including endocrine function and tumorigenesis.
 
Overall design single-nuclei RNA sequencing (snRNA-seq) of 3 APAs and 2 non-functional adenomas (NFA)
 
Contributor(s) Murakami M, Hara K, Ikeda K, Yamada T
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Submission date Sep 05, 2023
Last update date Dec 01, 2023
Contact name BIN WU
E-mail(s) bin.wu@immunogeneteqs.com
Phone +81-4-7124-6361
Organization name ImmunoGeneTeqs
Department Research
Street address No.2669 Yamazaki
City Noda
ZIP/Postal code 278-0022
Country Japan
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM7763166 NFA1 and APA1 (Sample 1, GEX)
GSM7763167 NFA2, APA2 and APA3 (Sample 2, GEX)
GSM7763168 NFA1 and APA1 (Sample 1, Hashtag)
Relations
BioProject PRJNA1013234

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE242404_RAW.tar 80.3 Mb (http)(custom) TAR (of TXT)
GSE242404_Supplemental_file.xlsx 13.3 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA

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