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| Status |
Public on Sep 06, 2023 |
| Title |
Antigen perception in T cells by long-term Erk and NFAT signaling dynamics [scRNAseq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Immune system threat detection hinges on T cells’ ability to perceive varying peptide major-histocompatibility complex (pMHC) antigens. As the Erk and NFAT pathways link T cell receptor engagement to gene regulation, their signaling dynamics may convey information about pMHC inputs. To test this idea, we developed a dual reporter mouse strain and a quantitative imaging assay that, together, enable simultaneous monitoring of Erk and NFAT dynamics in live T cells over day-long timescales as they respond to varying pMHC inputs. Both pathways initially activate uniformly across various pMHC inputs, but diverge only over longer (9+ hrs) timescales, enabling independent encoding of pMHC affinity and dose. These late signaling dynamics are decoded via multiple temporal and combinatorial mechanisms to generate pMHC-specific transcriptional responses. Our findings underscore the importance of long timescale signaling dynamics in antigen perception, and establish a framework for understanding T cell responses under diverse contexts.
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| Overall design |
CD8+ T cells were isolated from splenocytes and stimulated with 5 different pMHC conditions, or rested, for 30 hrs.
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| Contributor(s) |
Wither MJ, White WL, Pendyala S, Leanza PJ, Fowler D, Kueh HY |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 06, 2023 |
| Last update date |
Sep 06, 2023 |
| Contact name |
Hao Yuan Kueh |
| E-mail(s) |
kueh@uw.edu
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| Organization name |
University of Washington
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| Department |
Bioengineering
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| Lab |
Kueh Lab
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| Street address |
3720 15th Ave NE
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (1) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE242495 |
Antigen perception in T cells by long-term Erk and NFAT signaling dynamics |
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| Relations |
| BioProject |
PRJNA1013586 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE242494_Conditions_HTOs_HTObarcode-sequences.txt.gz |
406 b |
(ftp)(http) |
TXT |
| GSE242494_barcodes.tsv.gz |
65.4 Kb |
(ftp)(http) |
TSV |
| GSE242494_features.tsv.gz |
284.2 Kb |
(ftp)(http) |
TSV |
| GSE242494_matrix.mtx.gz |
102.9 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
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