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Series GSE242537 Query DataSets for GSE242537
Status Public on Dec 01, 2023
Title Rare intercellular material transfer as a confound to interpreting inner retinal neuronal transplantation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Intercellular cytoplasmic material transfer (MT) occurs between transplanted and developing photoreceptors and ambiguates cell origin identification in developmental, transdifferentiation, and transplantation experiments. Whether MT is a photoreceptor-specific phenomenon is unclear. Retinal ganglion cell (RGC) replacement, through transdifferentiation or transplantation, holds potential for restoring vision in optic neuropathies. During careful assessment for MT following human stem cell-derived RGC transplantation into mice, we identified RGC xenografts occasionally giving rise to labeling of donor-derived cytoplasmic, nuclear, and mitochondrial proteins within recipient Müller glia. Critically, nuclear organization is distinct between human and murine retinal neurons, which enables unequivocal discrimination of donor from host cells. MT was dependent on internal limiting membrane disruption, which is also required for retinal engraftment following transplantation. Our findings demonstrate that retinal MT is not unique to photoreceptors and challenge the isolated use of species-specific immunofluorescent markers for xenotransplant identification. Assessment for MT is critical when analyzing neuronal replacement interventions.
 
Overall design human ESC derived retinal ganglion cells were differentiated according to the small molecule-based method and purified on day 42 of differentiation. Purified RGCs then underwent single-cell RNA-seq according to the Drop-seq protocol.
 
Citation(s) 37802075
Submission date Sep 07, 2023
Last update date Dec 14, 2023
Contact name Weifeng Li
E-mail(s) lwf9302@gmail.com
Organization name Johns hopkins university
Street address 400 N Broadway
City Baltimore
State/province Maryland
ZIP/Postal code 21231
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (1)
GSM7765229 hRGC day42
Relations
BioProject PRJNA1013821

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Supplementary file Size Download File type/resource
GSE242537_RAW.tar 1.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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