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Series GSE242806 Query DataSets for GSE242806
Status Public on Nov 01, 2023
Title MicroRNA-mediated attenuation of branched-chain amino acid catabolism promotes ferroptosis in chronic kidney disease [CLIP-seq]
Organism Mus musculus
Experiment type Other
Summary Chronic kidney disease (CKD) complicates cisplatin-based chemotherapy of cancer patients. Here we investigate microRNA (miRNA)-regulated transcriptomic activity to unveil biological processes associated with cisplatin-induced kidney injury. Implementing chimeric-eCLIP-seq approach to a mouse model for cisplatin-induced CKD, we identify direct pairs of miRNA and their target messenger RNA in the injured kidney. We find a dedicated transcriptomic program directed by a group of miRNAs that alter metabolic pathways centered on mitochondria in the injured kidneys. Specifically, cisplatin-induced miRNA, miR-429-3p suppresses the mitochondria pathway that catalyzes branched-chain amino acid (BCAA), eventually leading to lipid peroxidation-dependent cell death, called ferroptosis. Thus, the identification of miRNA-429-3p-mediated stimulation of ferroptosis suggests a therapeutic potential for BCAA pathway modulation in ameliorating CKD and cisplatin-associated nephrotoxicity.
Overall design To investigate the miRNA effect on CKD, cisplatin induced CKD mouse and control mouse were prepared.
eCLIPseq (to obtain miRNA-mRNA interaction through Ago2 complex), small RNAseq (to obtain miRNA), and RNAseq (to obtain mRNA) were performed.
Contributor(s) Sone H, Lee TJ, Lee BR, Heo D, Oh S, Kwon S
Citation(s) 38016961
Submission date Sep 09, 2023
Last update date Dec 13, 2023
Contact name Sang-Ho Kwon
Phone 706-721-0379
Organization name Augusta Univeristy
Department Cellular Biology and Anatomy
Street address 1120 15th Street
City Augusta
State/province Georgia
ZIP/Postal code 30912
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM7770903 eCLIP, IP, Control 1
GSM7770904 eCLIP, IP, Control 2
GSM7770905 eCLIP, IP, Control 3
This SubSeries is part of SuperSeries:
GSE242809 MicroRNA-mediated attenuation of branched-chain amino acid catabolism promotes ferroptosis in chronic kidney disease.
BioProject PRJNA1014776

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Supplementary file Size Download File type/resource
GSE242806_RAW.tar 853.0 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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