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Series GSE242807 Query DataSets for GSE242807
Status Public on Nov 01, 2023
Title MicroRNA-mediated attenuation of branched-chain amino acid catabolism promotes ferroptosis in chronic kidney disease [miRNA-seq]
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Chronic kidney disease (CKD) complicates cisplatin-based chemotherapy of cancer patients. Here we investigate microRNA (miRNA)-regulated transcriptomic activity to unveil biological processes associated with cisplatin-induced kidney injury. Implementing chimeric-eCLIP-seq approach to a mouse model for cisplatin-induced CKD, we identify direct pairs of miRNA and their target messenger RNA in the injured kidney. We find a dedicated transcriptomic program directed by a group of miRNAs that alter metabolic pathways centered on mitochondria in the injured kidneys. Specifically, cisplatin-induced miRNA, miR-429-3p suppresses the mitochondria pathway that catalyzes branched-chain amino acid (BCAA), eventually leading to lipid peroxidation-dependent cell death, called ferroptosis. Thus, the identification of miRNA-429-3p-mediated stimulation of ferroptosis suggests a therapeutic potential for BCAA pathway modulation in ameliorating CKD and cisplatin-associated nephrotoxicity.
 
Overall design To investigate the miRNA effect on CKD, cisplatin induced CKD mouse and control mouse were prepared.
eCLIPseq (to obtain miRNA-mRNA interaction through Ago2 complex), small RNAseq (to obtain miRNA), and RNAseq (to obtain mRNA) were performed.
 
Contributor(s) Sone H, Lee TJ, Lee BR, Heo D, Oh S, Kwon S
Citation(s) 38016961
Submission date Sep 09, 2023
Last update date Dec 13, 2023
Contact name Sang-Ho Kwon
E-mail(s) kkwon@augusta.edu
Phone 706-721-0379
Organization name Augusta Univeristy
Department Cellular Biology and Anatomy
Street address 1120 15th Street
City Augusta
State/province Georgia
ZIP/Postal code 30912
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM7770915 Control, miRNA1
GSM7770916 Control, miRNA2
GSM7770917 Control, miRNA3
This SubSeries is part of SuperSeries:
GSE242809 MicroRNA-mediated attenuation of branched-chain amino acid catabolism promotes ferroptosis in chronic kidney disease.
Relations
BioProject PRJNA1014775

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE242807_ALL_MERGE_0_miRNA.txt.gz 10.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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