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Series GSE242886 Query DataSets for GSE242886
Status Public on Apr 21, 2024
Title Precise immuno-fluorescence canceling enables highly multiplexed imaging [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cell states are regulated by extrinsic signals from various external factors such as intercellular interactions, and intrinsic gene expression. Although comprehensive cell state profiling has been attempted, it remains simultaneous analysis of signal activation has still been challenging. Multiplexed imaging is a technique acquiring multiple protein information at a single cell level as traditional immunofluorescence. However, the method often compromises resolution, hindering the analysis of intracellular localization dynamics and post-translational modifications of proteins. To address these limitations, we developed an erasable fluorescence method using disulfide linkers to label antibodies. We term these antibodies ‘Precise Emission Canceling Antibodies (PECAbs)’. PECAb allows for high-resolution iterative imaging with minimal non-specific binding. Automation enables our system to achieve reproducible quantitative analysis using 206 antibodies. The resulting quantitative data allow reconstruction of the spatiotemporal dynamics of signaling pathways over both long and short timescales. Additionally, combining this approach with sequential RNA-FISH can effectively classify cells and identify their signal activation states in human tissue. Overall, the PECAb system serves as a comprehensive platform for analyzing complex cell processes, from signal transduction to gene expression.
 
Overall design To obtain carcinoma associated geneset from uterus carcinosarcoma tissue, three carcinosarcoma tissues containing different ratios of carcinoma:sarcoma (C:S = 3:7, 1:9 and 0:10) were used.
 
Contributor(s) Tomimatsu K, Takeru F, Tanaka K, Mori S, Ohkawa Y
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Submission date Sep 11, 2023
Last update date Apr 21, 2024
Contact name Yasuyuki Ohkawa
E-mail(s) yohkawa@bioreg.kyushu-u.ac.jp
Organization name Medical Institute of Bioregulation
Lab Division of Transcriptomics
Street address 3-1-1 Maidashi
City Fukuoka
ZIP/Postal code 8128582
Country Japan
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (3)
GSM7774385 C30S70_Rep1-3
GSM7774386 C10S90_Rep1-3
GSM7774387 C0S100_Rep1-3
This SubSeries is part of SuperSeries:
GSE242887 Precise immuno-fluorescence canceling enables highly multiplexed imaging
Relations
BioProject PRJNA1015232

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE242886_RawCountTable.tsv.gz 550.0 Kb (ftp)(http) TSV
GSE242886_sample_barcodes.txt.gz 190 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA

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