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Series GSE242923 Query DataSets for GSE242923
Status Public on Sep 12, 2023
Title miRNA expression profile from Adipocyte-derived Small Extracellular Vesicles from the Serum and cerebrospinal fluid of Alzheimer Disease Patients and Controls
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Midlife obesity increases the risk of developing AD. Adipocyte-derived small extracellular vesicles (ad-sEVs) have been implicated as a mecha-nism in several obesity-related diseases. We hypothesized that ad-sEVs from patients with AD would contain miRNAs predicted to downregulate pathways involved in synaptic plasticity and memory formation. We isolated ad-sEVs from the serum and cerebrospinal fluid (CSF) of patients with AD and controls and compared miRNA expression profiles
Overall design Ad-sEV miRNA was isolated from the serum (n=12 controls, n=17 AD) and cerebrospinal fluid (n=10 controls, n=14 AD). Total RNA was extracted using mirVana microRNA Isolation kits (Life Technologies). Total RNA was amplified with the Complete Seramir Exosome RNA Amplification Kit (System Biosciences, Mountainview, CA). RNA quality was assessed on a subset of these samples using the Agilent 2100 Bioanalyzer and nanodrop. RNA concentration was measured using a Qubit RNA Broad Range Assay Kit (Thermo Fisher Scientific, Waltham, MA). 1,000 ng of RNA was labeled with Affymetrix FlashTag Biotin HSR RNA Labeling Kit (Affymetrix, Santa Clara, CA) per the manufacturer’s instructions. Labeled RNA was hybridized to Affymetrix GeneChip microRNA 4.0 arrays. Chips were run using a Fluidics Station 450 Protocol (Affymetrix, Santa Clara, CA). The results were exported to Partek Genomics Suite for analysis.
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Contributor(s) Batabyal RA, Bansal A, Reck Cechinel L, Authelet K, Goldberg M, Nadler E, Keene CD, Jayadev S, Domoto-Reilly K, Li G, Peskind E, Hashimoto-Torii K, Buchwald D, Freishtat RJ
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Submission date Sep 11, 2023
Last update date Sep 24, 2023
Contact name Rachael Batabyal
Organization name Children's National Hospital
Street address 111 Michigan Ave NW
City Washington
ZIP/Postal code 20010
Country USA
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (53)
GSM7775050 CSF_AD_1
GSM7775051 CSF_AD_2
GSM7775052 CSF_AD_3
BioProject PRJNA1015352

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Supplementary file Size Download File type/resource
GSE242923_RAW.tar 39.9 Mb (http)(custom) TAR (of CEL)

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