|
| Status |
Public on May 10, 2024 |
| Title |
T cell mediated microglia activation triggers myelin pathology in a model of amyloidosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Age-related myelin damage induces inflammatory responses, yet its involvement in Alzheimer’s disease (AD) remains uncertain, despite age being a major risk factor. Using a mouse model of AD, we found that amyloidosis itself triggers age-related oligodendrocyte and myelin damage. Mechanistically, CD8+ T cells promote the progressive accumulation of abnormally interferon-activated microglia that display myelin-damaging activity. Thus, immune responses against myelinating oligodendrocytes may contribute to neurodegenerative diseases with amyloidosis.
|
| |
|
| Overall design |
To determine whether CD8+ T cells contribute to oligodendrocyte and myelin pathology in 5xFAD mice, we treated 6-months old 5xFAD mice with antibodies against CD8. For the depletion of CD8+ T cells mice, 5xFAD mice aged 6 months were intraperitoneally injected with anti-CD8 antibody (BioXCell, BP0061) and their respective isotype controls (BioXCell, BE0119) twice a week for a total of 6 weeks.For 10X genomic experiments, mice were deeply anesthetized and perfused with cold PBS. Each brain was removed and individually microdissected under a dissection microscope, wherein the cortex and hippocampus were isolated from the brain and the attached choroid plexus was removed.
|
| |
|
| Contributor(s) |
Feng R |
| Citation(s) |
38937583 |
| |
| Submission date |
Sep 12, 2023 |
| Last update date |
Aug 12, 2024 |
| Contact name |
Ruoqing Feng |
| E-mail(s) |
fengruoqing123@gmail.com
|
| Organization name |
German Center for Neurodegenerative Diseases (DZNE)
|
| Lab |
MikaLab
|
| Street address |
Feodor-Lynen-Strasse 17
|
| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA1015730 |
Less...
More...