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Series GSE243406 Query DataSets for GSE243406
Status Public on May 29, 2024
Title Unravelling the etiology of dilated cardiomyopathy through differential miRNA-mRNA interactome
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Dilated cardiomyopathy (DCM) entails a broad group of diseases, acquired or genetic, that share a similar phenotype. The understanding of gene-specific pathogenetic mechanisms and the determination of the functional effects of each variant may tailor different therapeutic strategies. MicroRNAs (miRNAs) are short sequences of non-coding RNA that play an important role in the development of several cardiovascular diseases, including DCM. Here, we applied mRNA and small RNA sequencing to identify the gene and miRNA signature from myocardial biopsies from four patients with DCM caused by volume overload (VCM) and four with ischemic DCM (ICM). Analysis showed five miRNAs and 112 mRNAs dysregulated in VCM vs ICM. Differentially expressed genes were positively enriched for extracellular matrix (ECM), mitochondria respiration-related, cardiac muscle contraction and fatty acid pathways in VCM vs ICM, and negatively enriched for immune response-related pathways, JAK-STAT and NF-kappa B signaling pathways in VCM vs ICM. miRNA-mRNA interaction analysis revealed four negatively correlated miRNA-target transcript pairs, miR-218-5p-DDX6, miR-218-5p-TT39C, miR-218-5p-SEMA4A and miR-494-3p-SGMS2. qRT-PCR validation showed a strong correlation between RNA-seq and qRT-PCR for these genes and miRNAs. These findings suggest that transcriptome signatures may distinguish distinct etiologies of DCM shedding light on underlying biological differences between VCM and ICM.
Overall design We used RNA-seq and small RNA-seq to identify etiology-specific transcriptome signatures in myocardial biopsies from volume overload dilated cardiomyopathy (VCM) and ischemic cardiomyopathy (ICM) patients without any valve disease
Contributor(s) Fernando B, Francisco H, Rocío T
Citation(s) 38785931
Submission date Sep 18, 2023
Last update date May 29, 2024
Contact name Francisco Hernandez-Torres
Phone +34 637833588
Organization name Universidad de Granada
Department Dept. of Biochemistry, Molecular Biology III and Immunology
Street address Avda. de la Investigación, 11
City Granada
State/province Granada
ZIP/Postal code 18016
Country Spain
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (15)
GSM7785095 21s004499
GSM7785096 21s004500
GSM7785097 21s004501
BioProject PRJNA1018441

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Supplementary file Size Download File type/resource
GSE243406_named_count_matrix.xlsx 2.2 Mb (ftp)(http) XLSX
GSE243406_named_count_matrix_miR.xlsx 86.1 Kb (ftp)(http) XLSX
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