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Series GSE243434 Query DataSets for GSE243434
Status Public on Sep 22, 2023
Title Nucleus-Independent Transgenerational Small RNA Inheritance in Caenorhabditis elegans
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary In C. elegans worms, epigenetic information transmits transgenerationally. Still, it is unknown whether the effects transfer to the next generation inside or outside of the nucleus. Here we use the tractability of gene-specific double-stranded RNA-induced silencing to demonstrate that RNAi can be inherited independently of any nuclear factors via mothers that are genetically engineered to transmit only their ooplasm but not the oocytes’ nuclei to the next generation. We characterize the mechanisms and, utilizing RNA sequencing, chimeric worms and sequence polymorphism between different isolates, identify endogenous small RNAs which, similarly to exogenous siRNAs, are inherited in a nucleus-independent manner. From an historical perspective, these results might be regarded as partial vindication of discredited cytoplasmic inheritance theories from the 19th century, such as Darwin’s “pangenesis” theory.
Overall design RNA was extracted from "day one" adult nematodes of different isolates (N2 and MY16). The samples include 3 biological replicates of worms in four conditions (MY16, MY16_star, N2, N2_star). 11 samples are from small RNA sequencing (one sample from MY16_star failed to yield sufficient RNA) and 12 samples are from total RNA sequencing, yielding a total of 23 samples.
Contributor(s) Rieger I, Weintraub G, Lev I, Goldstein K, Bar-Zvi D, Anava S, Gingold H, Shaham S, Rechavi O
Citation(s) 37878696
Submission date Sep 18, 2023
Last update date Nov 13, 2023
Contact name Hila Gingold
Organization name Tel-Aviv University
Street address 30 Haim Levanon Street
City Tel Aviv
ZIP/Postal code 6997801
Country Israel
Platforms (1)
GPL19757 Illumina NextSeq 500 (Caenorhabditis elegans)
Samples (23)
GSM7785576 MY16_sRNA_R1
GSM7785577 MY16_sRNA_R2
GSM7785578 MY16_sRNA_R3
BioProject PRJNA1018494

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE243434_count_table_mRNA.csv.gz 451.3 Kb (ftp)(http) CSV
GSE243434_count_table_sRNA_AS.csv.gz 422.4 Kb (ftp)(http) CSV
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Raw data are available in SRA

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