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Series GSE243509 Query DataSets for GSE243509
Status Public on May 24, 2024
Title TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity [03_scatac_shTRPS1_organoids]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The GATA transcription factor TRPS1 is a context-dependent oncogene in breast cancer. In the mammary gland, TRPS1 activity is restricted to the luminal population and sustains the initial branching of the mammary ducts at puberty as well as the lactogenic differentiation during pregnancy. Its function in the resting mammary gland however, remains unclear. To evaluate whether it could be a target for cancer therapy, we investigated TRPS1 function in the healthy resting mammary gland using a conditional ubiquitous depletion mouse model where long-term depletion does not affect fitness. we show that TRPS1 activity is critical to maintain a functional luminal progenitor compartment and that this involves the repression of the SRF/MRTF and the YAP/TAZ transcriptional program. Mechanistically, this repression involves Krt8-dependent RhoA modulation. Our work uncovers a hitherto undisclosed function of TRPS1 in luminal progenitors which is intrinsically linked to mechanotransduction in the mammary gland. It also provides new insights into the oncogenic functions of TRPS1 since luminal progenitors are likely the cells of origin of many breast cancers.
 
Overall design To analyze the impact of TRPS1 depletion on chromatin accessibility, primary mouse mammary gland organoids from shTRPS1 transgenic mice (n=4) were cultured and shRNA expression was induced by doxycycline treatment (DOX; 0.1 µg/ml). Ethanol-treated organoids (EtOH) served as a control.
 
Contributor(s) Tollot-Wegner M, Jessen M, Kim K, von Eyss B
Citation(s) 38702730
Submission date Sep 19, 2023
Last update date May 24, 2024
Contact name Bjoern von Eyss
E-mail(s) bjoern.voneyss@leibniz-fli.de
Organization name Leibniz Institute on Aging
Lab von Eyss
Street address Beutenbergstr. 11
City Jena
ZIP/Postal code 07745
Country Germany
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM7789728 shTRPS1 DOX
GSM7789729 shTRPS1 EtOH
This SubSeries is part of SuperSeries:
GSE243511 TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity
Relations
BioProject PRJNA1018830

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Supplementary file Size Download File type/resource
GSE243509_RAW.tar 432.8 Mb (http)(custom) TAR (of BED, MTX, TSV)
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Raw data are available in SRA

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