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Series GSE243567

Query DataSets for GSE243567

Status

Public on Sep 24, 2023

Title

Expression data from pulmonary artery endothelial cell after exposure to hypoxia for 24 hours

Organism

Homo sapiens

Experiment type

Expression profiling by array

Summary

A number of mediators that have been found to be involved in the pathogenesis of hypoxic pulmonary hypertension, these mediators including mRNA, microRNAs and long noncoding RNAs. Endothelial dysfunction plays a major role in the initiation of pulmonary vascular remodeling and is thought to be a major contributor to this process. However, mRNA and no-coding RNAs expression profiles and their biological functions in pulmonary artery endothelial cells (PAECs) exposed to hypoxia need to be investigated further.
Pulmonary artery endothelial cells (PAECs) were exposed to normoxia condition (21% O2, 5% CO2, 74% N2) or hypoxia condition (3% O2, 5% CO2, 92% N2) (n=3 for each group).PAECs were harvested after 24 hours and performed to further analysis.

Overall design

cDNAs of samples from normoxia and hypoxia group were hybridized to Affymetrix Clariom D human GeneChip® arrays (Affymetrix, America) according to the User Manuals. Affymetrix® Expression Console Software (version 1.2.1) was used for microarray analysis. Raw data (CEL files) were normalized at transcript level using robust multiaverage method (RMA workflow). Median summarization of transcript expressions was calculated. Gene-level data was then filtered to include only those probe sets that are in the ‘core’ metaprobe list, which represent RefSeq genes.

Contributor(s)

Liu J, Wang L

Citation(s)

37965157

Submission date

Sep 19, 2023

Last update date

Dec 07, 2023

Contact name

李玉琳

Organization name

Beijing Institute of Heart Lung and Blood Vessel Diseases