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Series GSE243567 Query DataSets for GSE243567
Status Public on Sep 24, 2023
Title Expression data from pulmonary artery endothelial cell after exposure to hypoxia for 24 hours
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A number of mediators that have been found to be involved in the pathogenesis of hypoxic pulmonary hypertension, these mediators including mRNA, microRNAs and long noncoding RNAs. Endothelial dysfunction plays a major role in the initiation of pulmonary vascular remodeling and is thought to be a major contributor to this process. However, mRNA and no-coding RNAs expression profiles and their biological functions in pulmonary artery endothelial cells (PAECs) exposed to hypoxia need to be investigated further.
Pulmonary artery endothelial cells (PAECs) were exposed to normoxia condition (21% O2, 5% CO2, 74% N2) or hypoxia condition (3% O2, 5% CO2, 92% N2) (n=3 for each group).PAECs were harvested after 24 hours and performed to further analysis.
Overall design cDNAs of samples from normoxia and hypoxia group were hybridized to Affymetrix Clariom D human GeneChip® arrays (Affymetrix, America) according to the User Manuals. Affymetrix® Expression Console Software (version 1.2.1) was used for microarray analysis. Raw data (CEL files) were normalized at transcript level using robust multiaverage method (RMA workflow). Median summarization of transcript expressions was calculated. Gene-level data was then filtered to include only those probe sets that are in the ‘core’ metaprobe list, which represent RefSeq genes.
Contributor(s) Liu J, Wang L
Citation(s) 37965157
Submission date Sep 19, 2023
Last update date Dec 07, 2023
Contact name 李 玉琳
Organization name Beijing Institute of Heart Lung and Blood Vessel Diseases
Street address 2 Anzhen Rd, Chaoyang District,
City Beijing
ZIP/Postal code 100029
Country China
Platforms (1)
GPL23126 [Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version]
Samples (6)
GSM7791173 PAECs, normoxia condition, rep1
GSM7791174 PAECs, normoxia condition, rep2
GSM7791175 PAECs, normoxia condition, rep3
BioProject PRJNA1018943

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE243567_RAW.tar 127.8 Mb (http)(custom) TAR (of CEL, CHP)

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