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Series GSE243751 Query DataSets for GSE243751
Status Public on Oct 02, 2023
Title Epithelial Ovarian Cancer is infiltrated by activated effector T cells co-expressing CD39, PD-1, TIM-3, CD137 and interacting with cancer cells and myeloid cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Introduction: Despite predicted efficacy, immunotherapy in epithelial ovarian cancer (EOC) has limited clinical benefit and the prognosis of patients remains poor. There is thus a strong need for better identifying local immune dynamics and immune-suppressive pathways limiting T-cell mediated anti-tumor immunity. Methods: In this observational study we analyzed by immunohistochemistry, gene expression profiling and flow cytometry the antigenic landscape and immune composition of 48 EOC specimens, with a focus on tumor-infiltrating lymphocytes (TILs). Results: Activated T cells showing features of partial exhaustion with a CD137+CD39+PD-1+TIM-3+CD45RA-CD62L-CD95+ surface profile were exclusively present in EOC specimens but not in corresponding peripheral blood or ascitic fluid, indicating that the tumor microenvironment might sustain this peculiar phenotype. Interestingly, while neoplastic cells expressed several tumor-associated antigens possibly able to stimulate tumor-specific TILs, macrophages provided both co-stimulatory and inhibitory signals and were more abundant in TILs-enriched specimens harboring the CD137+CD39+PD-1+TIM-3+CD45RA-CD62L-CD95+ signature. Conclusions: These data demonstrate that EOC is enriched in CD137+CD39+PD-1+TIM-3+CD45RA-CD62L-CD95+ T lymphocytes, a phenotype possibly modulated by antigen recognition on neoplastic cells and by a combination of inhibitory and co-stimulatory signals largely provided by infiltrating myeloid cells. Furthermore, we have identified immunosuppressive pathways potentially hampering local immunity which might be targeted by immunotherapeutic approaches
Overall design 46 samples derived from epithelial ovarian cancer patients were analyzed
Contributor(s) Tassi E, Bergamini A, Wignall J, Sant’Angelo M, Brunetto E, Balestrieri C, Bonini C
Citation(s) 37868997
Submission date Sep 21, 2023
Last update date Oct 25, 2023
Contact name Chiara Balestrieri
Organization name IRCCS San Raffaele Scientific Institute
Department Center for Omics Sciences
Street address Via Olgettina 58
City Milan
ZIP/Postal code 20132
Country Italy
Platforms (1)
GPL31236 Customized nCounter PanCancer IO 360 Panel [770 + 20]
Samples (46)
GSM7795282 Patient#1
GSM7795283 Patient#2
GSM7795284 Patient#3
BioProject PRJNA1019744

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Supplementary file Size Download File type/resource
GSE243751_RAW.tar 450.0 Kb (http)(custom) TAR (of RCC)
GSE243751_normalized.txt.gz 164.1 Kb (ftp)(http) TXT

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