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Series GSE24385 Query DataSets for GSE24385
Status Public on Dec 23, 2010
Title NAKR1 deletion analysis
Organism Arabidopsis thaliana
Experiment type Genome variation profiling by genome tiling array
Summary ABSTRACT:The SODIUM POTASSIUM ROOT DEFECTIVE 1 (NaKR1) encodes a soluble metal binding protein that is specifically expressed in companion cells of the phloem. The nakr1-1 mutant phenotype includes high Na+, K+, Rb+ and starch accumulation in leaves, short roots, late flowering and decreased long-distance transport of sucrose. Using traditional and DNA microarray-based mapping, a 7 bp deletion was found in an exon of NaKR1 that caused a premature stop. The mutant phenotypes were complemented by the native gene and by GFP and GUS fusions driven by the native promoter. NAKR1-GFP was mobile in the phloem, it moved into sieve elements and into a novel symplasmic domain of the root meristem. Grafting experiments revealed that the high Na+ accumulation was due primarily to loss of NaKR1 function in the leaves. This supports a role for the phloem in recirculating Na+ to the roots to limit Na+ accumulation in leaves. The onset of root phenotypes coincided with NaKR1 expression after germination. Short root length was primarily due to a decrease in cell division rate in the root meristem indicating a role for NaKR1 expression in the phloem in root meristem maintenance.
 
Overall design 3 hybridizations each of mutant NAKR1 and wildtype for deletion identification.
 
Citation(s) 21193571
Submission date Sep 27, 2010
Last update date May 09, 2017
Contact name Ivan Baxter
E-mail(s) ibaxter@danforthcenter.org
Organization name USDA-ARS
Department PLant Genetics Research Unit
Lab Baxter
Street address 975 North Warson Rd
City St. Louis
State/province MO
ZIP/Postal code 63132
Country USA
 
Platforms (1)
GPL10978 [At35b_MF] Affymetrix Arabidopsis Tiling 1.0F Array
Samples (6)
GSM601145 Col-0_tiling_hyb1
GSM601146 Col-0_tiling_hyb2
GSM601147 Col-0_tiling_hyb3
Relations
BioProject PRJNA132871

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24385_RAW.tar 197.6 Mb (http)(custom) TAR (of CEL)
Processed data not provided for this record

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