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Series GSE244158 Query DataSets for GSE244158
Status Public on Oct 02, 2023
Title Gene expression analysis of sorted murine B16-F10 melanoma tumor derived endothelial cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Individualized outcome prediction classifiers were successfully constructed through expression profiling of a total of 8,644 genes in 50 non-small cell lung cancer (NSCLC) cases, which had been consecutively operated on within a defined short period of ti
Overall design 250,000 B16-F10 melanoma cells were subcutaneously implanted in the right flank of C57B6 mice (WT and ATG5-BECKO). Tumors were digested 18 days post grafting using collagenase digestion. From digested tumors, immune cells were depleted by Miltenyi magnetic beads labelled with anti CD45 and subsequently endotheilal cells were enriched by Miltentyi magantic beads labelled with anti CD31. The cell pellets obtained after enrichment of CD31+ endothelial cells was stained with fluroscently labelled antiobodies and pure endothelial cells were sorted as Live CD45-CD31+ cells.
Contributor(s) Patrizia A
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Submission date Sep 27, 2023
Last update date Oct 02, 2023
Contact name Patrizia Agostinis
Phone 003216-330650
Organization name VIB-KU Leuven
Department Cellular and Molecular Medicine
Lab Laboratory of Cell Death Research & Therapy
Street address Herestraat 49 - box 802
City Leuven
ZIP/Postal code 3000
Country Belgium
Platforms (1)
GPL28689 NanoString nCounter Mouse PanCancer IO 360 Panel [NS_Mm_IO_360_v1.0]
Samples (6)
GSM7807973 SAMPLE 1_WT
BioProject PRJNA1021549

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Supplementary file Size Download File type/resource
GSE244158_RAW.tar 60.0 Kb (http)(custom) TAR (of RCC)

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