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Series GSE244227 Query DataSets for GSE244227
Status Public on Oct 03, 2023
Title Effect of blocking A2aR in sepsis on the Treg transcriptome
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In our study, blocking A2aR with ZM241385 was found to inhibit Foxp3 expression in Treg cells of septic mice. Therefore, we used flow cytometry to sort mouse spleen CD4+CD25+ cells for transcriptome sequencing in an attempt to discover the mechanism by which blocking A2aR affects Foxp3 expression. The specific groupings were as follows: naive groups (N1-N5), CLP groups (C1-C5), CLP+A2aR antagonist groups (Z1-Z6).
Overall design This part of the data consists of the following three sections: splenic Treg cells from normal mice, splenic Treg cells from mice with sepsis CLP model, and splenic Treg cells from mice with sepsis CLP model treated with ZM241385.The Treg cells were labeled with CD4+CD25+ antibody and sorted using flow cytometry.
Contributor(s) Zhang T, Ma T
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Submission date Sep 28, 2023
Last update date Oct 03, 2023
Contact name Teng Zhang
Organization name Tianjin Medical University General Hospital
Street address nanjing road
City tianjin
ZIP/Postal code 300052
Country China
Platforms (1)
GPL28330 DNBSEQ-T7 (Mus musculus)
Samples (16)
GSM7809086 splenic Treg cells, C1
GSM7809087 splenic Treg cells, C2
GSM7809088 splenic Treg cells, C3
BioProject PRJNA1021818

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Supplementary file Size Download File type/resource
GSE244227_processeddata.txt.gz 2.0 Mb (ftp)(http) TXT
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Raw data are available in SRA

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