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| Status |
Public on Oct 03, 2023 |
| Title |
Effect of blocking A2aR in sepsis on the Treg transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In our study, blocking A2aR with ZM241385 was found to inhibit Foxp3 expression in Treg cells of septic mice. Therefore, we used flow cytometry to sort mouse spleen CD4+CD25+ cells for transcriptome sequencing in an attempt to discover the mechanism by which blocking A2aR affects Foxp3 expression. The specific groupings were as follows: naive groups (N1-N5), CLP groups (C1-C5), CLP+A2aR antagonist groups (Z1-Z6).
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| Overall design |
This part of the data consists of the following three sections: splenic Treg cells from normal mice, splenic Treg cells from mice with sepsis CLP model, and splenic Treg cells from mice with sepsis CLP model treated with ZM241385.The Treg cells were labeled with CD4+CD25+ antibody and sorted using flow cytometry.
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| Contributor(s) |
Zhang T, Ma T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 28, 2023 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Teng Zhang |
| E-mail(s) |
zhttj@live.cn
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| Organization name |
Tianjin Medical University General Hospital
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| Street address |
nanjing road
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| City |
tianjin |
| ZIP/Postal code |
300052 |
| Country |
China |
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| Platforms (1) |
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| Samples (16)
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| GSM7809089 |
splenic Treg cells, C4 |
| GSM7809090 |
splenic Treg cells, C5 |
| GSM7809091 |
splenic Treg cells, N1 |
| GSM7809092 |
splenic Treg cells, N2 |
| GSM7809093 |
splenic Treg cells, N3 |
| GSM7809094 |
splenic Treg cells, N4 |
| GSM7809095 |
splenic Treg cells, N5 |
| GSM7809096 |
splenic Treg cells, Z1 |
| GSM7809097 |
splenic Treg cells, Z2 |
| GSM7809098 |
splenic Treg cells, Z3 |
| GSM7809099 |
splenic Treg cells, Z4 |
| GSM7809100 |
splenic Treg cells, Z5 |
| GSM7809101 |
splenic Treg cells, Z6 |
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| Relations |
| BioProject |
PRJNA1021818 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE244227_processeddata.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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