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| Status |
Public on Sep 28, 2023 |
| Title |
Transcriptomic response of Mycobacterium tuberculosis to artemisinin, Artemisia annua extract, and Artemisia afra extract |
| Organism |
Mycobacterium tuberculosis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We sought to determine the transcriptomic impacts of artemisinin, Artemisia annua extract, and Artemisia afra extract on M. tuberculosis. Log phase cultures were treated with lethal doses for four hours or with inhibitory or sub-inhibitory doses for 24 hours. RNA was collected from untreated controls at the same timepoint.
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| Overall design |
M. tuberculosis strain mc26230 (H37Rv ΔRD1 ΔpanCD) was treated with artemisinin or with dichloromethane extracts of A. annua or A. afra at three different doses. A lethal dose (2X MIC) was applied for four hours, and inhibitory (MIC or 1.33X MIC) and sub-inhibitory doses (0.5X MIC) were applied for 24 hours. Untreated cultures were harvested after 4 and 24 hours for comparison. All treatments were done in quadruplicate.
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| Contributor(s) |
Shell SS, Alonso MN, Xiao J |
| Citation(s) |
37873198 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R21 AI151481 |
The mechanistic basis of Artemisia annua activity against Mycobacterium tuberculosis |
WORCESTER POLYTECHNIC INSTITUTE |
Scarlet Sara Shell |
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| Submission date |
Sep 28, 2023 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Scarlet Shell |
| E-mail(s) |
sshell@wpi.edu
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| Organization name |
Worcester Polytechnic Institute
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| Department |
Biology and Biotechnology
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| Lab |
Shell Lab
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| Street address |
60 Prescott St, WPI
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| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
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| Platforms (1) |
| GPL25317 |
Illumina HiSeq 4000 (Mycobacterium tuberculosis) |
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| Samples (44)
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| Relations |
| BioProject |
PRJNA1021956 |
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