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Series GSE24479 Query DataSets for GSE24479
Status Public on Aug 01, 2012
Title Evolution of S. cerevisiae - S. bayanus Interspecific Hybrids
Platform organisms Saccharomyces bayanus; Saccharomyces cerevisiae
Sample organisms Saccharomyces bayanus; Saccharomyces cerevisiae; Saccharomyces bayanus x Saccharomyces cerevisiae
Experiment type Genome variation profiling by array
Summary Hybrid progeny can enjoy increased fitness and stress tolerance relative to their ancestral species, a phenomenon known as hybrid vigor. Though this phenomenon has been documented throughout the Eukarya, evolution of hybrid populations has yet to be explored experimentally in the lab. To fill this knowledge gap we created a pool of Saccharomyces cerevisiae and S. bayanus homoploid and aneuploid hybrids, and then investigated how selection in the form of incrementally increased temperature or ethanol impacted hybrid genome structure and adaptation. During 500 generations of continuous ammonia-limited, glucose-sufficient culture, temperature was raised from 25C to 46??C. This selection invariably resulted in nearly-complete loss of the S. bayanus genome, although the dynamics of genome loss differed among independent replicates. Temperature-evolved isolates were significantly more thermal tolerant and exhibited greater phenotypic plasticity than parental species and founding hybrids. By contrast, when the same hybrid pool was subjected to increases in exogenous ethanol from 0% to 14%, selection favored euploid S. cerevisiae x S. bayanus hybrids. Ethanol-evolved isolates exhibited significantly greater ethanol tolerance relative only to S. bayanus and one of the founding hybrids tested. Adaptation to thermal and ethanol stress manifested as heritable changes in cell wall structure demonstrated by resistance to zymolyase or micafungin treatment. This is the first study to show experimentally that the fate of interspecific hybrids critically depends on the type of selection they encounter during the course of evolution.
 
Overall design Array-CGH was performed on the S. cerevisiae parent strain CEN.PK (GSY2160), the S. bayanus parent strain CBS7001 (GSY2161) and on the F1 interspecific hybrid resulting from mating the 2 parents (GSY2168). Additionally, three rare viable spores obtained after sporulation of the F1 were assayed by array-CGH (F2a, F2b, F2c). A large pool of F2 spores (and probably some number of F1 hybrid cells) were subjected to gradually increasing temperatures, in three independent vessels, with populations sampled at various generation times. Likewise, the same pool was used to found populations in an additional three independent vessels, which were then subjected to gradually increasing ethanol concentrations (at constant temperature). Array-CGH was performed on three different clones from each of the three temperature vessels at the final 500 generation time point (T500 clones). Biological replicates of the T500 clones were performed (T500-new). Two self-self array-CGH hybridization controls were also performed (self-control). Array-CGH was performed on one clone from each of the three ethanol vessels taken at the 400 generation timepoint (EtOH400gen clones).
 
Contributor(s) Dunn B
Citation(s) 22471618
Submission date Oct 01, 2010
Last update date Nov 30, 2012
Contact name Barbara Dunn
E-mail(s) bdunn@stanford.edu
Phone 650-498-5995
Organization name Stanford University
Department Genetics
Street address -
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL11007 Agilent SMD Print_1600 Saccharomyces bayanus and Saccharomyces cerevisiae
Samples (29)
GSM820672 T500-Cc
GSM820673 T500-Ca
GSM820674 T500-Cc-New
Relations
BioProject PRJNA132509

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24479_RAW.tar 570.0 Kb (http)(custom) TAR
Processed data included within Sample table

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