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GEO help: Mouse over screen elements for information. |
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Status |
Public on Nov 05, 2023 |
Title |
Gene expression profile at single cell level of J558 allograft mouse model treated with the CDC7-specific inhibitor TAK-931 [scRNA-seq] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To comprehensively evaluate in vivo inflammatory effects of TAK-931 in tumor microenvironment, scRNA-seq was conducted in the J558 allograft mouse model. The tSNE plots and the expression dot plots showed cellular components in the allografts classified by the indicated TME markers: cancer cell (J558 allograft), monocyte, CD8+ T cell, CD4+ T cell, NK cell, endothelial cell, fibroblast, and undefined, demonstrating that TAK-931 treatment prominently promoted tumor infiltration of both adaptive (CD8+ T cell and CD4+ T cell) and innate (NK cells and monocytes) immune cells in the allografts.
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Overall design |
The J558-allografted BALB/c female mice were orally administered vehicle or TAK-931 at 60 mg/kg, QD for 6 days. One sample from each group was used for scRNA-seq.
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Contributor(s) |
Kashima Y, Mashima C, Morita TY, Yoshiya M, Ohashi A |
Citation(s) |
37980406 |
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Submission date |
Oct 10, 2023 |
Last update date |
Dec 08, 2023 |
Contact name |
Akihiro Ohashi |
E-mail(s) |
aohashi@east.ncc.go.jp
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Phone |
+81-4-7133-1111
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Organization name |
National Cancer Center Japan
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Department |
Exploratory Oncology Research and Clinical Center
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Lab |
Division of Collaborative Research and Development
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Street address |
6-5-1 Kashiwanoha
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City |
Kashiwa |
State/province |
Chiba |
ZIP/Postal code |
2778577 |
Country |
Japan |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (2) |
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Relations |
BioProject |
PRJNA1026570 |
Supplementary file |
Size |
Download |
File type/resource |
GSE244986_RAW.tar |
120.9 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
Raw data are available in SRA |
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