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Series GSE244986 Query DataSets for GSE244986
Status Public on Nov 05, 2023
Title Gene expression profile at single cell level of J558 allograft mouse model treated with the CDC7-specific inhibitor TAK-931 [scRNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To comprehensively evaluate in vivo inflammatory effects of TAK-931 in tumor microenvironment, scRNA-seq was conducted in the J558 allograft mouse model. The tSNE plots and the expression dot plots showed cellular components in the allografts classified by the indicated TME markers: cancer cell (J558 allograft), monocyte, CD8+ T cell, CD4+ T cell, NK cell, endothelial cell, fibroblast, and undefined, demonstrating that TAK-931 treatment prominently promoted tumor infiltration of both adaptive (CD8+ T cell and CD4+ T cell) and innate (NK cells and monocytes) immune cells in the allografts.
Overall design The J558-allografted BALB/c female mice were orally administered vehicle or TAK-931 at 60 mg/kg, QD for 6 days. One sample from each group was used for scRNA-seq.
Contributor(s) Kashima Y, Mashima C, Morita TY, Yoshiya M, Ohashi A
Citation(s) 37980406
Submission date Oct 10, 2023
Last update date Dec 08, 2023
Contact name Akihiro Ohashi
Phone +81-4-7133-1111
Organization name National Cancer Center Japan
Department Exploratory Oncology Research and Clinical Center
Lab Division of Collaborative Research and Development
Street address 6-5-1 Kashiwanoha
City Kashiwa
State/province Chiba
ZIP/Postal code 2778577
Country Japan
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM7833259 J558 allograft, Vehicle, Day 6
GSM7833260 J558 allograft, TAK-931, Day 6
BioProject PRJNA1026570

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Supplementary file Size Download File type/resource
GSE244986_RAW.tar 120.9 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA

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