GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE24519 Query DataSets for GSE24519
Status Public on Dec 31, 2017
Title Gene expression profiling of patients affected by first acute myocardial infarction (FAMI)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background: Myocardial infarction (MI) is the single most critical event in coronary disease. Platelets partecipate in processes that precipitate acute MI, lack nuclear DNA but retain megakaryocyte mRNAs, thus their transcriptome could provide information preceding the coronary event. Our aim was to obtain a gene expression atlas of platelets from patients with their very first acute MI (FAMI) in order to identify potential markers of precipitating factors.
Methods and results: Platelets from seventeen FAMI patients and matched controls were collected and RNA and protein were purified. Expression profiles were obtained with genome-wide microarrays. Platelet-specific proteomic analysis was also performed with fluorescent DIGE separation in the same samples. Strong transcriptional down-regulation was observed in patient platelets, whereas more balanced numbers of up and down-represented proteins were found by proteomic analysis.
Conclusions: Platelet transcriptome revealed quantitative differences between FAMI patients and controls. The functional analysis deregulated mRNAs and the integration with proteomic data in platelets may suggest pre-activation of platelets and/or megakaryocytes in FAMI patients before the acute event.
Overall design We have selected a group of seventeen patients affected by their very first acute myocardial infarction (FAMI), without any sign of previous cardiovascular sufferance. Whole blood was collected within 6 hours from the acute event and freshly purified platelets were used to profile their mRNA population by DNA microarrays. We performed microarray experiments using the CodeLink Expression Bioarray System (GE Healthcare). Total RNA (1.5 µg) was amplified and biotin-labeled using the CodeLink Expression Assay Reagent Kit following the manufacturer’s protocol. Microarray data analysis identified 712 specifically modulated transcripts, out of 17,594, in FAMI patients, compared with normal controls; the majority (666) was down-regulated, and only 46 genes resulted up-regulated. The main result was that all the patients share some common mechanism that leads to a massive and statistical relevant transcript down-regulation.
Contributor(s) Bellin M, Nanni L, De Pittà C, Viganò A, Vallanti G, Vasso M, Vozzi D, Cristell N, Cianflone D, Gelfi C, Maseri A, Lanfranchi G
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 05, 2010
Last update date Dec 31, 2017
Contact name Gerolamo Lanfranchi
Phone +39-0498276219
Organization name University of Padova
Department CRIBI - Biotechnology Center and Biology Department
Lab Functional Genomics Lab
Street address Via U. Bassi, 58/B
City Padova
ZIP/Postal code 35131
Country Italy
Platforms (1)
GPL2895 GE Healthcare/Amersham Biosciences CodeLink Human Whole Genome Bioarray
Samples (38)
GSM604559 FAMI patient N. 15 (A)
GSM604560 FAMI patient N. 15 (B)
GSM604561 FAMI patient N. 16 (A)
This SubSeries is part of SuperSeries:
GSE24591 Gene and microRNA expression profiling of patients affected by first acute myocardial infarction (FAMI)
BioProject PRJNA133517

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap